...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Phytochemical Analysis >Quantitative analysis of five kudinosides in the large-leaved Kudingcha and related species from the genus Ilex by UPLC-ELSD.
【24h】

Quantitative analysis of five kudinosides in the large-leaved Kudingcha and related species from the genus Ilex by UPLC-ELSD.

机译：用UPLC-ELSD定量分析大叶苦丁茶和相关属中五种苦丁子苷的含量。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction - The large-leaved Kudingcha from the genus Ilex, which is used as a traditional Chinese tea, contains several characteristic triterpenoid saponins that can be subjected to quality control evaluation. Objective - To develop and validate a rapid method incorporating reverse-phase ultra-performance liquid chromatography coupled with evaporative light scattering detection (UPLC-ELSD) for the simultaneous determination of the five triterpenoid saponins kudinoside L (1), kudinoside C (2), kudinoside A (3), kudinoside F (4) and kudinoside D (5) in several species of the large-leaved Kudingcha from the genus Ilex and 'Yerba Mate' (Ilex paraguariensis). Methodology - The five compounds were separated using a water-acetonitrile mobile phase with a Waters Acquity BEH C18-column (100x2.1 mm, 1.7 micro m). Results - Separation took 13 min with detection and quantification limits ranging from 12.5 to 29.8 ng and 41.3 to 98.2 ng, respectively. The method was validated according to the regulatory guidelines with respect to precision, stability, repeatability and recovery. The triterpenoid saponins showed a good regression relationship (r2
机译：简介-来自Ilex属的大叶苦丁茶，被用作传统中国茶，其中包含几种特征性三萜皂苷，可以进行质量控制评估。目的-建立并验证一种结合反相超高效液相色谱和蒸发光散射检测（UPLC-ELSD）的快速方法，用于同时测定五种三萜皂苷苦参皂苷L（1），苦参皂苷C（2），在Ilex和'Yerba Mate'属（Ilex paraguariensis）的大叶Kudingcha的几种物种中，存在苦参皂苷A（3），苦参皂苷F（4）和苦参皂苷D（5）。方法-用水-乙腈流动相和Waters Acquity BEH C 18 柱（100x2.1 mm，1.7微米）分离五种化合物。结果-分离进行了13分钟，检测和定量限分别为12.5至29.8 ng和41.3至98.2 ng。该方法已根据监管准则在精度，稳定性，可重复性和回收率方面进行了验证。三萜皂苷在测试范围内具有良好的回归关系（r 2

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号