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Protein nanotubes bearing a magnetite surface exterior

机译:带有磁铁矿表面外部的蛋白质纳米管

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This communication describes the synthesis and structure of multilayered protein nanotubes bearing a magnetite (Fe_3O_4) surface exterior, and their binding capability for zinc(II)-protoporphyrin IX (ZnPP) in aqueous medium. The nanotubes were fabricated using an alternating layer-by-layer (LbL) assembly of human serum albumin (HSA) and poly-L-arginine (PLA) in a track-etched polycarbonate (PC) membrane (pore size, 400 nm), which had been precoated in advance with Fe_3O_4 nanoparticles. Dissolution of the PC template yielded Fe_3O_4(PLA/HSA)_3 nanotubes. SEM measurements revealed the formation of uniform hollow cylinders with 417±16 nm outer diameter and 56±7 nm wall thickness. TEM observations confirmed the homogeneous outer surface of Fe_3O_4. In an aqueous medium, the nanotubes captured ZnPP into the swollen wall. The ZnPP-loaded protein nanotubes were collected by exposure to a magnetic field.
机译:该交流描述了具有磁铁矿(Fe_3O_4)表面外部的多层蛋白质纳米管的合成和结构,以及它们在水性介质中对锌(II)-原卟啉IX(ZnPP)的结合能力。使用人血清白蛋白(HSA)和聚L-精氨酸(PLA)的交替逐层(LbL)组件在轨迹蚀刻的聚碳酸酯(PC)膜(孔径为400 nm)中制造纳米管,预先用Fe_3O_4纳米颗粒进行了预涂。溶解PC模板产生Fe_3O_4(PLA / HSA)_3纳米管。 SEM测量表明形成了具有417±16nm外径和56±7nm壁厚的均匀空心圆柱体。 TEM观察证实了Fe_3O_4的均匀外表面。在水性介质中,纳米管将ZnPP捕获到溶胀的壁中。通过暴露于磁场中来收集负载ZnPP的蛋白质纳米管。

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