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Methionine and selenium yeast supplementation of the maternal diets affects antioxidant activity of breeding eggs.

机译:孕妇饮食中补充蛋氨酸和硒酵母会影响种蛋的抗氧化活性。

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摘要

Four hundred fifty 52-wk-old Langshan layer hens (dual-purpose type, an indigenous poultry breed of China) were randomly divided into 9 treatments with 5 replicates in each treatment. Birds were fed corn-soybean diets (0.13 mg of Se/kg) supplemented with 0, 0.30, and 0.60 mg/kg of Se from Se yeast and 3.2, 4.0, and 5.4 g of DL-Met/kg, respectively. Increasing Se yeast supplementation significantly increased Se concentration in the egg yolk (P < 0.01) and the Se concentration of the 3.2 g of Met/kg treatment was higher than those of the 4.0 and 5.4 g of Met/kg treatments. Adding 0.3 mg of Se/kg to the diet significantly increased glutathione peroxidase (GSH-Px) activity in the egg yolk compared with 0 and 0.6 mg of Se/kg (P < 0.01) and increasing Se yeast supplementation significantly increased the GSH-Px activity in the egg albumen (P < 0.01). Increasing Met supplementation significantly decreased the GSH-Px activity in both the yolk and the albumen of the eggs (P < 0.01). Methionine supplemented at 3.2 and 4.0 g/kg significantly increased glutathione concentration in the egg yolk compared with 5.4 g of Met/kg (P < 0.01) and increasing Met supplementation increased the glutathione concentration in the egg albumen. Increasing Met supplementation significantly decreased malondialdehyde concentration in the egg yolk (P < 0.01) and Se supplemented at 0 and 0.6 mg/kg increased the malondialdehyde concentrations in the egg yolk compared with 0.3 mg of Se/kg (P < 0.01). Methionine supplemented at 4.0 and 5.4 g/kg significantly decreased carbonyl concentration compared with 3.2 g of Met/kg. The conclusion was drawn that Se yeast and Met supplementation of the maternal diets could enhance antioxidant activity of breeding eggs.
机译:将450只52周龄的Lang山蛋鸡(两用型,中国本土家禽品种)随机分为9个处理,每个处理重复5次。饲喂玉米-大豆日粮(0.13 mg Se / kg),分别补充了来自Se酵母的0、0.30和0.60 mg / kg Se和3.2、4.0和5.4 g DL-Met / kg。硒酵母添加量的增加显着提高了蛋黄中硒的含量(P <0.01),3.2 g Met / kg处理的硒浓度高于4.0和5.4 g Met / kg处理的硒浓度。日粮中添加0.3 mg Se / kg的蛋黄比0和0.6 mg Se / kg的蛋黄中的谷胱甘肽过氧化物酶(GSH-Px)活性显着增加(P <0.01),并且增加Se酵母的添加量会显着增加GSH-Px卵清蛋白的活性(P <0.01)。蛋氨酸和蛋清蛋白中蛋氨酸和蛋清中Met含量的增加显着降低了GSH-Px的活性(P <0.01)。与5.4 g Met / kg相比,蛋黄中添加3.2和4.0 g / kg的蛋氨酸显着增加了谷胱甘肽的浓度(P <0.01),并且Met的添加增加了蛋清中谷胱甘肽的浓度。增加Met的添加显着降低了蛋黄中的丙二醛浓度(P <0.01),而添加0和0.6 mg / kg的Se则增加了蛋黄中的丙二醛浓度,而Se / kg为0.3 mg(P <0.01)。与3.2 g Met / kg相比,补充4.0和5.4 g / kg的蛋氨酸可显着降低羰基浓度。得出的结论是,硒酵母和Met补充母体饮食可以增强种蛋的抗氧化活性。

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