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Effects of dietary n-6:n-3 ratio on immune and reproductive systems of pullet chicks

机译:日粮n-6:n-3比例对雏鸡免疫和生殖系统的影响。

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The objective of this study was to examine the effects of dietary n-6:n-3 ratio on immune and reproduction systems of Leghorn pullet chicks. A total of 216 Hy-Line W-36 pullet chicks (1 d old) were randomly assigned to 3 diets (n-6:n-3 ratios of 10, 6, and 2) until 22 wk of age. The Optomega-50 (Optivite International Ltd., Nottinghamshire, UK) containing 50% salmon fish oil was used as an n-3 source. Pullets were injected with SRBC suspension at 6 and 9 wk and anti-SRBC titers were measured 7 and 14 d after each immunization. The antibody (Ab) titers for Newcastle disease (ND), avian influenza, infectious bronchitis (IB), and infectious bursal disease (IBD) were determined at 4, 6, 10, 14, and 18 wk of age. Pullets fed diet with the n-6:n-3 ratio of 2 had higher feed consumption and lower BW gain (P < 0.05) compared those fed diets with the ratios of 6 and 10. The results demonstrated that the different dietary n-6:n-3 ratios did not have a significant effect on the anti-SRBC titers in pullets serum (P > 0.05). The Ab production against avian influenza vaccine was increased in pullets fed diet containing the n-6:n-3 ratio of 10 at wk 10 and 14 and increased for ND at only 14 wk (P < 0.05). On the other hand, the Ab response to IBD (14 wk) and IB (14 wk) vaccines was increased when pullets were fed diet containing a ratio of 6 (P < 0.05). The pullets fed diet with the n-6:n-3 ratio of 2 showed lower egg production and late sexual maturity (P > 0.05) whereas ovary weight (P = 0.01) and number of large yellow follicles (P = 0.049) were significantly decreased at first oviposition. Our results revealed that the supplementation of Optomega-50 as a source of n-3 to decrease the n-6:n-3 ratio in diet significantly increased Ab production for ND, IB, and IBD in pullet chicks with slight reduction in reproductive organs weight at early production.
机译:这项研究的目的是检验日粮n-6:n-3比例对来克霍恩小鸡免疫和生殖系统的影响。总共216只Hy-Line W-36母鸡(1天大)被随机分配到3种饮食中(n-6:n-3的比例分别为10、6和2),直到22周龄。含有50%鲑鱼鱼油的Optomega-50(英国诺丁汉郡的Optivite国际有限公司)被用作n-3来源。免疫后第6周和第9周给小鸡注射SRBC悬浮液,并在第7和14天测量抗SRBC滴度。确定新城疫(ND),禽流感,传染性支气管炎(IB)和传染性法氏囊病(IBD)的抗体(Ab)滴度在4、6、10、14和18周龄。饲喂n-6:n-3比例为2的小鸡与饲喂6和10比例的那些相比,具有较高的饲料消耗和较低的体重增加(P <0.05)。结果表明,不同的饮食n-6 :n-3比值对小鸡血清中的抗SRBC滴度没有显着影响(P> 0.05)。在第10周和第14周时,n-6:n-3比率为10的小母鸡饲喂的抗禽流感疫苗的Ab产量增加,而在第14周时的ND则增加(P <0.05)。另一方面,当母鸡饲喂比例为6的日粮时,对IBD(14 wk)和IB(14 wk)疫苗的抗体反应增加。以n-6:n-3比为2的日粮饲喂的小母鸡显示出较低的产卵量和性成熟后期(P> 0.05),而卵巢重量(P = 0.01)和大黄色卵泡数量(P = 0.049)显着初产时下降。我们的结果表明,补充Optomega-50作为n-3的来源以降低日粮中n-6:n-3的比例可显着提高雏鸡ND,IB和IBD的Ab产生,而生殖器官则略有减少生产初期的重量。

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