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首页> 外文期刊>Poultry Science >Immunological responses to Clostridium perfringens alpha-toxin in two genetically divergent lines of chickens as influenced by major histocompatibility complex genotype.
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Immunological responses to Clostridium perfringens alpha-toxin in two genetically divergent lines of chickens as influenced by major histocompatibility complex genotype.

机译：受主要组织相容性复合体基因型影响的两种遗传分化鸡品系中产气荚膜梭菌α毒素的免疫反应。

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Chickens genetically selected for low (LA) or high (HA) antibody response to SRBC displayed a correlated change in MHC, so that LA chickens were 96% B13 and HA chickens were 96% B21. The LA line appears to be less susceptible to invasion by extracellular pathogens, whereas HA chickens are more resistant to infection by intracellular organisms. Resistance to Clostridium perfringens is one instance in which the lines do not follow their established trend of pathogen susceptibility, where during a clinical outbreak of necrotic enteritis, B21B21 genotypes experienced significantly less mortality than B13B13 genotypes. A study was carried out to assess immunological differences between LA and HA lines during exposure to C. perfringens alpha -toxin. Peripheral blood mononuclear cells were isolated from each genetic line, cultured with or without lipopolysaccharide (4 h), and exposed to varying concentrations of alpha -toxin (1; 10; 100; and 1,000 U/L) for 2 and 4 h. Evaluation of cellular proliferation, percentage of cytotoxicity, and immunological gene expression was carried out in a series of experiments. Cells isolated from HA chickens had significantly increased proliferation than those from LA chickens at low toxin levels (1 and 10 U/L) and significantly decreased proliferation at high toxin levels (100 and 1,000 U/L). Following exposure to lipopolysaccharide, the percentage of cytotoxicity was higher for LA than HA cells. In both assays, HA cells displayed superior performance following lipopolysaccharide-stimulation. Gene expression analysis of immune transcripts by quantitative real-time PCR revealed significantly upregulated expression of interferon (IFN)- gamma , interleukin (IL)-8, IL-13 (2 h), IL-15, and CXCLi1 (4 h) in HA than LA chickens. Cells isolated from the LA line displayed significantly elevated expression of IL-2, IL-10, IL-13 (4 h), IL-16, IL-18, inducible nitric oxide synthase (iNOS), CXCLi1 (2 h), and lipopolysaccharide-induced tumor necrosis factor- alpha factor (LITAF) compared with the HA line. Clearly, these 2 genetic lines display highly divergent immune responses in regards to C. perfringens toxin exposure.
机译：经过遗传选择对SRBC具有低（LA）或高（HA）抗体反应的鸡，其MHC发生了相关变化，因此LA鸡的B13为96％，HA鸡的B21为96％。 LA品系似乎不易受到细胞外病原体的侵袭，而HA鸡对细胞内生物体的感染更具抵抗力。对产气荚膜梭状芽孢杆菌的抗性是其中品系不遵循病原体易感性的既定趋势的一种情况，其中在临床坏死性肠炎暴发期间，B21B21基因型的死亡率显着低于B13B13基因型。进行了一项研究，以评估暴露于iC期间LA和HA品系之间的免疫学差异。产气菌α-毒素。从每个遗传系中分离外周血单个核细胞，在有或没有脂多糖的条件下培养（4 h），并暴露于不同浓度的α-毒素（1; 10; 100;和1,000 U / L）下2和4 h。在一系列实验中对细胞增殖，细胞毒性百分比和免疫基因表达进行了评估。在低毒素水平（1和10 U / L）下，从HA鸡中分离的细胞比从LA鸡中分离出的细胞显着增加，而在高毒素水平（100和1,000 U / L）下，其细胞则明显降低。暴露于脂多糖后，LA的细胞毒性百分比高于HA细胞。在两种测定中，HA细胞在脂多糖刺激后均表现出优异的性能。实时荧光定量PCR对转录本的基因表达分析表明，干扰素（ IFN ）-γ，白介素（ IL ） -8 ， IL-13 （2 h）， IL-15 和 CXCLi1 （4 h）在LA鸡中。从LA系分离的细胞显示 IL-2 ， IL-10 ， IL-13 （4 h）， IL-16 ， IL-18 ，诱导型一氧化氮合酶（ iNOS ）， CXCLi1 （2 h）和脂多糖诱导的肿瘤坏死因子-α因子（ LITAF ）与HA系相比。显然，这两个遗传系显示出关于C的高度不同的免疫反应。产气荚膜毒素的暴露。

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