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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Genetic characterization of pPHDP60, a novel conjugative plasmid from the marine fish pathogen Photobacterium damselae subsp. piscicida
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Genetic characterization of pPHDP60, a novel conjugative plasmid from the marine fish pathogen Photobacterium damselae subsp. piscicida

机译:pPHDP60的遗传特性,这是一种来自海洋鱼类病原体damselae亚种的新型共轭质粒。 sc

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摘要

A new plasmid designated pPHDP60 from a strain of the marine bacterium Photobacterium damselae subsp. piscicida isolated from diseased seabream has been characterised. pPHDP60 consists of 59,731. bp, has a G. +. C content of 37.2% and encodes 63 predicted open-reading frames (ORFs). The plasmid backbone sequence includes, among other genes, 15 ORFs homologous to proteins of type IV conjugation systems described in IncP-type plasmids. Two modules could be distinguished within pPHDP60 sequence. One module included 10 genes of a putative type II secretion system with homologues in other Photobacterium and Vibrio plasmids. A second module exhibiting a transposon structure included a functional haloalkane dehalogenase gene linB as well as a toxin/antitoxin system. Additional interesting features of pPHDP60 include its ability to be conjugally transferred to several Gram negative bacteria.
机译:来自海洋细菌光细菌damselae亚种的菌株的新质粒命名为pPHDP60。从患病的鲷鱼中分离出的piscicida已被鉴定。 pPHDP60由59,731组成。 bp,有一个G。 C含量为37.2%,编码63个预测的开放阅读框架(ORF)。除其他基因外,质粒主链序列还包括与IncP型质粒中所述的IV型缀合系统蛋白同源的15个ORF。在pPHDP60序列中可以区分两个模块。一个模块包括推定的II型分泌系统的10个基因,该基因在其他细菌和弧菌质粒中具有同源性。展示转座子结构的第二个模块包括功能性卤代烷脱卤酶基因linB以及毒素/抗毒素系统。 pPHDP60的其他有趣特征包括其可共轭转移至几种革兰氏阴性细菌的能力。

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