Arginine-rich RNA binding domain and protein scaffold domain of RNase E are important for degradation of RNAI but not for that of the Rep mRNA of the ColE2 plasmid.

Nishio SY; Itoh T

首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Arginine-rich RNA binding domain and protein scaffold domain of RNase E are important for degradation of RNAI but not for that of the Rep mRNA of the ColE2 plasmid.

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Arginine-rich RNA binding domain and protein scaffold domain of RNase E are important for degradation of RNAI but not for that of the Rep mRNA of the ColE2 plasmid.

机译：RNase E的富含精氨酸的RNA结合结构域和蛋白质支架结构域对RNAI的降解很重要，但对ColE2质粒的Rep mRNA的降解却不重要。

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Expression of the replication initiator protein (Rep) of the ColE2 plasmid is controlled by antisense RNA (RNAI). Therefore alterations in processes and/or rates of degradation of these two RNAs would affect the Rep expression. Here, we have shown that the arginine-rich RNA binding domain (ARRBD) of RNase E is important for the initial endoribonucleolytic cleavage of RNAI but dispensable for the endoribonucleolytic cleavages of the Rep mRNA. We have also shown that the protein scaffold domain of RNase E is important for successive exoribonucleolytic degradation of RNAI, suggesting involvement of RhlB, but dispensable for that of the Rep mRNA. Such differences in the initiation and successive steps of degradation between RNAI and the Rep mRNA might be important in determining their individual degradation efficiencies required for a quick response to the changes in the plasmid copy number.

机译：ColE2质粒的复制起始蛋白（Rep）的表达受反义RNA（RNAI）的控制。因此，这两个RNA的过程和/或降解速率的改变将影响Rep表达。在这里，我们已经显示，RNase E的富含精氨酸的RNA结合结构域（ARRBD）对于RNAI的初始核糖核酸内切酶裂解很重要，但对于Rep mRNA的核糖核酸内切酶裂解是必不可少的。我们还表明，RNase E的蛋白质支架结构域对于连续的核糖核酸外切核糖核酸降解至关重要，提示RhlB参与其中，但对于Rep mRNA却是必不可少的。 RNAI和Rep mRNA之间的降解起始步骤和后续降解步骤之间的这种差异可能对确定对质粒拷贝数变化的快速响应所需的它们各自的降解效率很重要。

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《Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems》 |2009年第2期|共5页
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Nishio SY; Itoh T;
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正文语种 eng
中图分类医学遗传学;
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1. Arginine-rich RNA binding domain and protein scaffold domain of RNase E are important for degradation of RNAI but not for that of the Rep mRNA of the ColE2 plasmid. [J] . Nishio SY, Itoh T Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2009,第2期

机译：RNase E的富含精氨酸的RNA结合结构域和蛋白质支架结构域对RNAI的降解很重要，但对ColE2质粒的Rep mRNA的降解却不重要。
2. Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation. [J] . Raynal LC, Carpousis AJ Molecular Microbiology . 1999,第4期

机译：大肠杆菌的Poly（A）聚合酶I：表征催化结构域，RNA结合位点和与参与mRNA降解的蛋白质相互作用的区域。
3. A KH Domain RNA Binding Protein, KSRP, Promotes ARE-Directed mRNA Turnover by Recruiting the Degradation Machinery [J] . Roberto Gherzi, Kyung-Yeol Lee, Paola Briata, Molecular cell . 2004,第5期

机译：KH域RNA结合蛋白KSRP通过招募降解机器来促进ARE导向的mRNA转换
4. UV-induced protein-RNA cross-linking: Identification of RNA-binding domains, peptides, and amino acids [C] . Katharina Kramer, Timo Sachsenberg, Oliver Kohlbacher, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：UV诱导的蛋白质RNA交联：鉴定RNA结合结构域，肽和氨基酸
5. Characterization of two RNA-binding proteins containing RNA-Recognition Motif (RRM)-type RNA-binding domains from mouse brain. [D] . Inman, Michael Verne. 1999

机译：表征两个RNA结合蛋白的小鼠大脑中的RNA识别基序（RRM）型RNA结合域。
6. Control of ColE2 DNA replication: in vitro binding of the antisense RNA to the Rep mRNA. [O] . T Sugiyama, T Itoh 1993

机译：ColE2 DNA复制的控制：反义RNA与Rep mRNA的体外结合。
7. Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation [O] . Lelia C. Raynal, Agamemnon J. Carpousis 1999

机译：大肠杆菌的聚（A）聚合酶I：催化结构域的表征，RNA结合位点和与参与mRNA降解的蛋白质相互作用的区域

Arginine-rich RNA binding domain and protein scaffold domain of RNase E are important for degradation of RNAI but not for that of the Rep mRNA of the ColE2 plasmid.

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