首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Complete sequence and analysis of the stability functions of pPSX, a vector that allows stable cloning and expression of Streptomycete genes in Escherichia coli K12.

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Complete sequence and analysis of the stability functions of pPSX, a vector that allows stable cloning and expression of Streptomycete genes in Escherichia coli K12.

机译：pPSX稳定功能的完整序列和分析，该载体可在大肠杆菌K12中稳定克隆和表达链霉菌基因。

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The broad host range, cloning and expression vector pPSX has been completely sequenced and analysed. pPSX is 14.7kb in length and contains the fusion of two continuous segments of the parental 34kb, IncW plasmid pR388. pPSX appears to have retained at least three sets of gene/s which contribute in different ways to plasmid stability. The first of these parB, is a known participant in the partitioning of low-copy number plasmids. While the adjoining gene, orf35, has high homology with kfrA, a putative plasmid nucleoid organiser that is often associated with the ParAB family of proteins. The second set of genes; orfs18, 19, 20, whose exact functions are not clear, have homology to the stability operons of both IncW and IncN plasmids. The third is the resolvase, resP, which may resolve plasmid multimers that can lead to plasmid instability. pPSX is a small, stable cloning vector good for cloning and expression of a wide range of genes, including those from streptomycetes.

机译：广泛的宿主范围，克隆和表达载体pPSX已被完全测序和分析。 pPSX的长度为14.7kb，包含亲本34kb IncW质粒pR388的两个连续片段的融合体。 pPSX似乎保留了至少三组基因，它们以不同的方式对质粒的稳定性作出了贡献。这些parB的第一个是低拷贝数质粒分配中的已知参与者。虽然相邻的基因orf35与kfrA具有高度同源性，kfrA是一种推定的质粒类核苷组织者，通常与ParAB蛋白家族相关。第二组基因; orfs18、19、20的确切功能尚不清楚，与IncW和IncN质粒的稳定操纵子具有同源性。第三个是resolvase resP，它可以解析质粒多聚体，从而导致质粒不稳定。 pPSX是一种小型，稳定的克隆载体，可用于克隆和表达各种基因，包括来自链霉菌的基因。

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《Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems》 |2009年第1期|共5页
作者
Philip DS; Sarovich DS; Pemberton JM;
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正文语种 eng
中图分类医学遗传学;
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1. Complete sequence and analysis of the stability functions of pPSX, a vector that allows stable cloning and expression of Streptomycete genes in Escherichia coli K12. [J] . Philip DS, Sarovich DS, Pemberton JM Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2009,第1期

机译：pPSX稳定功能的完整序列和分析，该载体可在大肠杆菌K12中稳定克隆和表达链霉菌基因。
2. pPSY: a vector for the stable cloning and expression of streptomycete single gene phenotypes in Escherichia coli. [J] . Philip DS, Sarovich DS, Pemberton JM Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2008,第1期

机译：pPSY：用于在大肠杆菌中稳定克隆和表达链霉菌单基因表型的载体。
3. The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes [J] . D M Parker, I G Fotheringham, M E Finlay, The biochemical journal . 1986,第3期

机译：大肠杆菌K12中aspC和tyrB基因的克隆和序列分析。大肠杆菌天门冬氨酸氨基转移酶和芳香族氨基转移酶与猪天门冬氨酸氨基转移酶同工酶一级结构的比较
4. Sequence analysis of functional Apisimin-2 cDNA from royal jelly of Chinese honeybee and its expression in Escherichia coli [C] . Lirong Shen, Liping Xing, Yuxiao Yang, . 2006

机译：中国蜂王浆中功能性Apisimin-2 cDNA的序列分析及其在大肠杆菌中的表达
5. Heterodimeric deoxyguanosine/deoxyadenosine kinase from Lactobacillus acidophilus R-26: Affinity protein purification, molecular cloning, sequence of the genes, and expression in Escherichia coli. [D] . Ma, Grace Tak-Yi. 1993

机译：嗜酸乳杆菌R-26的异二聚体脱氧鸟苷/脱氧腺苷激酶：亲和蛋白纯化，分子克隆，基因序列和在大肠杆菌中的表达。
6. The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II the aroL-gene product. [O] . G Millar, A Lewendon, M G Hunter, 1986

机译：大肠杆菌K12的aroL基因的克隆和表达。 aroL基因产物sh草酸激酶II的纯化和完整氨基酸序列。
7. The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product. [O] . Millar, G, Lewendon, A, Hunter, M G, 1986

机译：大肠杆菌K12的aroL基因的克隆和表达。 aroL基因产物sh草酸激酶II的纯化和完整氨基酸序列。

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