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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >A novel plasmid for detection of N-acyl homoserine lactones.
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A novel plasmid for detection of N-acyl homoserine lactones.

机译：用于检测N-酰基高丝氨酸内酯的新型质粒。

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摘要

Many bacteria utilize acyl-homoserine lactones as cell to cell signals that can regulate the expression of numerous genes. Structural differences in acyl-homoserine lactones produced by different bacteria, such as acyl side chain length and the presence or absence of an oxy group, make many of the commonly used detection bioassays impractical for broad range detection. Here we present a simple, broad range acyl-homoserine lactone detection bioassay that can be used to detect a wide range of these chemical signals. A plasmid (pEAL01) was constructed and transformed into Pseudomonas aeruginosa strain QSC105 to allow for detection of a broad range of acyl-homoserine lactones through induction of a lasB'-lacZ transcriptional fusion. Monitoring beta-galactosidase activity from this bioassay showed that P. aeruginosa strain QSC105 (pEAL01) could detect the presence of eight acyl-homoserine lactones tested at physiological concentrations. This novel strain could also detect acyl-homoserine lactones from the extracts of four different bacteria that produce different acyl-homoserine lactones signals. These data indicate that strain QSC105 (pEAL01) can be used to detect a wide variety of acyl-homoserine lactones by a simple beta-galactosidase assay and this bioassay could be a useful and inexpensive tool to quickly identify the presence of these signal molecules.
机译：许多细菌利用酰基高丝氨酸内酯作为细胞间信号，可以调节众多基因的表达。由不同细菌产生的酰基-高丝氨酸内酯的结构差异，例如酰基侧链长度和是否存在氧基，使得许多常用的检测生物测定法不适合大范围检测。在这里，我们提出了一种简单的，范围广泛的酰基高丝氨酸内酯检测生物测定法，该方法可用于检测各种化学信号。构建质粒（pEAL01），并将其转化到铜绿假单胞菌菌株QSC105中，从而可以通过诱导lasB'-lacZ转录融合来检测广泛的酰基高丝氨酸内酯。监测此生物测定法中的β-半乳糖苷酶活性表明，铜绿假单胞菌菌株QSC105（pEAL01）可以检测到8种在生理浓度下测试的酰基高丝氨酸内酯的存在。该新型菌株还可以从产生不同酰基-高丝氨酸内酯信号的四种不同细菌的提取物中检测出酰基-高丝氨酸内酯。这些数据表明，菌株QSC105（pEAL01）可通过简单的β-半乳糖苷酶测定法用于检测各种酰基高丝氨酸内酯，该生物测定法可能是一种有用且便宜的工具，可快速识别这些信号分子的存在。

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