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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >THE RESISTANCE AND INTEGRASE GENES OF PACM1, A CONJUGATIVE MULTIPLE-RESISTANCE PLASMID, FROM KLEBSIELLA OXYTOCA
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THE RESISTANCE AND INTEGRASE GENES OF PACM1, A CONJUGATIVE MULTIPLE-RESISTANCE PLASMID, FROM KLEBSIELLA OXYTOCA

机译：缩合性克雷伯氏菌PACM1的抗性和整合基因

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摘要

pACM1 is an 85-kb conjugative plasmid from a clinical isolate of Klebsiella oxytoca that encodes resistance to beta-lactams (mediated by SHV-5 extended spectrum beta-lactamase), trimethoprim, sulfonamides, tetracycline, aminoglycosides, and mercuric chloride. The expression of the aminoglycoside resistance is difficult to detect, which could have clinical implications. A region of pACM1 containing five resistance genes and two putative integrons was characterized by restriction mapping and partial DNA sequencing. One integron appears to be class I (sulI type); the second lacks a recognizable 3' conserved segment. Neither integron has the BamHI site predicted for the 5' conserved segment. Plasmids encoding SHV-5 from other bacterial strains appear to be closely related to pACM1 by restriction enzyme analysis, but have resistance/integron regions that vary in size and content from that of pACM1. Integrase-mediated recombination might be responsible for genetic divergence in a widely distributed family of pACM1-like plasmids. (C) 1997 Academic Press. [References: 31]
机译：pACM1是来自产酸克雷伯菌的临床分离株的85 kb结合质粒，编码对β-内酰胺类（由SHV-5扩展谱β-内酰胺酶介导），甲氧苄啶，磺酰胺，四环素，氨基糖苷和氯化汞的抗性。氨基糖苷耐药性的表达难以检测，可能具有临床意义。通过限制酶切图谱和部分DNA测序来表征包含五个抗性基因和两个推定的整合素的pACM1区域。一个整合子似乎是I类（sulI型）;第二个缺少可识别的3'保守片段。两个整合子都没有预测5'保守区段的BamHI位点。通过限制酶分析，编码来自其他细菌菌株的SHV-5的质粒似乎与pACM1密切相关，但其抗性/整合子区域的大小和含量与pACM1不同。整合酶介导的重组可能负责广泛分布的pACM1样质粒家族中的遗传差异。 （C）1997学术出版社。 [参考：31]

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