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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >IDENTIFICATION OF PLASMIDS IN THE GENUS MARINOCOCCUS AND COMPLETE NUCLEOTIDE SEQUENCE OF PLASMID PPL1 FROM MARINOCOCCUS HALOPHILUS
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IDENTIFICATION OF PLASMIDS IN THE GENUS MARINOCOCCUS AND COMPLETE NUCLEOTIDE SEQUENCE OF PLASMID PPL1 FROM MARINOCOCCUS HALOPHILUS

机译：鉴定海洋球菌属中的质粒和来自盐球菌的质粒PPL1的完整核苷酸序列

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摘要

Several plasmids were detected in the Gram-positive halophilic eubacterium Marinococcus halophilus and in the related strain M52. The complete nucleotide sequence (3874 bp) of one of these plasmids, pPL1, was determined. Four major open reading frames were identified. Whereas orf3 and orf4 showed no sequence similarities to known proteins, rep displayed a high sequence similarity to replication proteins of rolling circle plasmids. Upstream of this ORE a sequence resembling the double-strand origin was detected. A region probably constituting the single-strand origin was identified. The ORF mob showed sequence similarity with Mob proteins of rolling circle plasmids. The observed characteristics suggest that pPL1 replicates according to the rolling circle mechanism. (C) 1998 Academic Press. [References: 32]
机译：在革兰氏阳性嗜盐性真细菌嗜盐球菌和相关菌株M52中检测到一些质粒。确定了其中一个质粒pPL1的完整核苷酸序列（3874 bp）。确定了四个主要的开放阅读框。 orf3和orf4与已知蛋白没有序列相似性，而rep与滚环质粒的复制蛋白却具有很高的序列相似性。在此ORE的上游，检测到类似于双链起源的序列。确定了可能构成单链起源的区域。 ORF mob与滚环质粒的Mob蛋白表现出序列相似性。观察到的特征表明pPL1根据滚环机理复制。 （C）1998年学术出版社。 [参考：32]

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