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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >CLONING AND CHARACTERIZATION OF THE ORI REGION OF PSW1200 OF ERWINIA STEWARTII - SIMILARITY WITH PLASMID P1
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CLONING AND CHARACTERIZATION OF THE ORI REGION OF PSW1200 OF ERWINIA STEWARTII - SIMILARITY WITH PLASMID P1

机译：斯威氏酵母PSW1200 ORI区的克隆与鉴定-与质粒P1相似。

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摘要

The ori region of an Erwinia stewartii plasmid, pSW1200 (106 kb), has been cloned and sequenced. This region consists of a gene encoding a protein which has 91% similarity and 73% identity with the RepA protein of bacteriophage P1. The ori region also consists of eight copies of 19-bp iterons which are highly homologous to the iterons of P1. Similar to plasmid P1, pSW1200 replicon has a copy number of approximately 1. On the other hand, the copy number increases about ninefold if three of the iterons located downstream from repA gene are deleted. We also demonstrate that pGEM-5Z consisting of a copy of P1 iteron is incompatible with a pSW1200 derivative, pSW1201, suggesting that pSW1200 and P1 DNA are incompatible and both belong to the IncY group. (C) 1997 Academic Press. [References: 18]
机译：Stewartii Erwinia stewartii质粒pSW1200（106 kb）的ori区已被克隆并测序。该区域由编码与噬菌体P1的RepA蛋白具有91％相似性和73％同一性的蛋白的基因组成。 ori区域也由八个拷贝的19bp的Iteron组成，这些拷贝与P1的Iteron高度同源。与质粒P1相似，pSW1200复制子的拷贝数约为1。另一方面，如果删除了repA基因下游的三个迭代子，则拷贝数将增加约9倍。我们还证明了由P1 iteron拷贝组成的pGEM-5Z与pSW1200衍生物pSW1201不兼容，这表明pSW1200和P1 DNA不兼容，并且都属于IncY组。 （C）1997学术出版社。 [参考：18]

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