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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >CTn12256, a chimeric Bacteroides conjugative transposon that consists of two independently active mobile elements.
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CTn12256, a chimeric Bacteroides conjugative transposon that consists of two independently active mobile elements.

机译：CTn12256，一种嵌合的拟杆菌共轭转座子，由两个独立活性的移动元件组成。

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摘要

A Bacteroides conjugative transposon (CTn), CTn12256, which has an estimated size of more than 150 kbp appeared to contain most or all of a previously discovered 65 kbp CTn, CTnDOT. To determine whether the integrated CTnDOT was still intact and to identify the element into which CTnDOT had integrated, large segments of CTn12256 were cloned and sequenced. Results of this analysis revealed that an intact CTnDOT type CTn had integrated into another CTn that was most closely related to a putative CTn found in the genome sequence of Bacteroides fragilis YCH46 (CTn3Bf). The CTnDOT portion of CTn12256 (CTnDOT2) proved to be capable of excising from CTn12256 and to transfer itself independently of CTn12256. The CTn3Bf region rarely transferred independently of CTnDOT2, and the transferred CTn3Bf contained a large deletion flanking the site of CTnDOT2 insertion and was no longer transmissible. Nonetheless, genetic analysis showed that the CTn3Bf portion controls transfer of CTn12256.
机译：估计大小超过150 kbp的拟杆菌共轭转座子（CTn），似乎包含了以前发现的65 kbp CTnDOT的大部分或全部。为了确定整合的CTnDOT是否仍然完好无损并确定整合了CTnDOT的元件，克隆了大段CTn12256并进行了测序。分析结果表明，完整的CTnDOT型CTn已整合到另一个CTn中，该CTn与脆弱拟杆菌（Bacteroides fragilis）YCH46（CTn3Bf）的基因组序列中发现的CTn最紧密相关。事实证明，CTn12256的CTnDOT部分（CTnDOT2）能够从CTn12256切除，并能够独立于CTn12256进行转移。 CTn3Bf区域很少独立于CTnDOT2转移，并且转移的CTn3Bf包含一个较大的缺失，位于CTnDOT2插入位点的侧面，并且不再可传播。尽管如此，遗传分析表明，CTn3Bf部分控制着CTn12256的转移。

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