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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Mobile genetic elements of Fusobacterium nucleatum.
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Mobile genetic elements of Fusobacterium nucleatum.

机译：核梭菌的移动遗传元件。

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The gram-negative anaerobic bacterium, Fusobacterium nucleatum, is a predominant member of the human oral flora. As a major component of subgingival plaque, this bacterium has a significant impact on the ecology of the oral cavity due to its ability to adhere to many different microbial species. The objective of this study was to identify and characterize plasmids and transposons that may have the potential to be developed into tools for cloning, genetic transformation, and mutagenesis of oral isolates of F. nucleatum. Analysis of a collection of laboratory strains resulted in the identification of a homologous family of small cryptic plasmids. Plasmids within this family ranged in size from 6.0 to 6.6 kb. Eighteen percent of all strains examined (n = 74) contained DNA sequences related to the plasmids. Homologous plasmid sequences were found in strains belonging to 2 of the 3 subspecies of the bacterium. The 2 smallest plasmid species were cloned in Escherichia coli to facilitate endonuclease restriction mapping. Among the strains examined for plasmids, 5 exhibited resistance to at least 10 micrograms/ml of tetracycline. These strains, all members of the subsp. polymorphum, contained a tetracycline resistance determinant (TetM) as part of a Tn916-like integrated transposon sequence. The Tn916-like element and 1 of the plasmid species co-resided in a single strain of the bacterium. Hybridization patterns of the Tn916-like sequences were identical in all 5 tetracycline-resistant strains. However, these strains appeared to be clonally distinct based on genomic fingerprinting.
机译：革兰氏阴性厌氧菌核梭菌是人类口腔菌群的主要成员。作为牙龈下菌斑的主要成分，这种细菌对口腔生态学有重大影响，因为它具有粘附许多不同微生物的能力。这项研究的目的是鉴定和表征质粒和转座子，这些质粒和转座子有可能被开发成用于克隆，遗传转化和诱变口腔念珠菌口腔分离物的工具。对实验室菌株集合的分析导致鉴定了小型隐性质粒的同源家族。该家族中的质粒大小范围为6.0至6.6 kb。检查的所有菌株中有18％（n = 74）包含与质粒相关的DNA序列。在属于该细菌的3个亚种中的2个的菌株中发现了同源质粒序列。将2种最小的质粒种类克隆到大肠杆菌中以促进核酸内切酶限制性图谱分析。在检查质粒的菌株中，有5个对至少10微克/毫升的四环素具有抗性。这些菌株，亚种的所有成员。多形体包含四环素抗性决定簇（TetM），作为Tn916样整合转座子序列的一部分。 Tn916样元件和1个质粒种类共存于单个细菌菌株中。在所有5个四环素抗性菌株中，Tn916样序列的杂交模式相同。然而，基于基因组指纹图谱，这些菌株在克隆上似乎是不同的。

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