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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Molecular characterization of a plasmid-borne (pTC82) chloramphenicol resistance determinant (cat-TC) from Lactobacillus reuteri G4.
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Molecular characterization of a plasmid-borne (pTC82) chloramphenicol resistance determinant (cat-TC) from Lactobacillus reuteri G4.

机译：罗伊氏乳杆菌G4的质粒介导的（pTC82）氯霉素抗性决定簇（cat-TC）的分子表征。

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摘要

Lactobacillus reuteri G4 contains a 7.0-kb plasmid (pTC82) encoding resistance to chloramphenicol (Cm). Determination of the nucleotide sequence of the genetic determinant (cat-TC) encoding resistance to Cm on pTC82 revealed an open reading frame for a 238-amino-acid Cm acetyltransferase (CAT) monomer. This structural cat gene, 714 bp in length, was highly related (ca. 95% nucleotide and ca. 81% amino acid identity) to the 648-bp cat gene from Staphylococcus aureus plasmid pC194. A total of 6 bp transversions and 4 bp deletions was observed along the whole DNA sequence of cat-TC compared to that of cat-pC194. To determine the activity of the putative cat-TC gene, recombinant plasmid pUC8217 containing the cat determinant from pTC82 was subjected to a maxicell analysis. The observed molecular mass of the synthesized protein, based on electrophoretic mobility, was in reasonable agreement with the 27.3 kDa predicted from the DNA sequence. This is the first reported nucleotide sequence of a Cm-resistance determinant from L. reuteri and also the first evidence of adding Lactobacillus to the list of versatile bacterial genera which naturally acquire the cat-pC194 gene in the microbial ecological system.
机译：罗伊氏乳杆菌G4含有一个7.0 kb的质粒（pTC82），编码对氯霉素（Cm）的抗性。在pTC82上编码抗Cm的遗传决定子（cat-TC）核苷酸序列的确定揭示了一个238个氨基酸的Cm乙酰转移酶（CAT）单体的开放阅读框。该结构猫基因长度为714 bp，与金黄色葡萄球菌质粒pC194的648-bp cat基因高度相关（约95％核苷酸和约81％氨基酸同一性）。与cat-pC194的整个DNA序列相比，沿着cat-TC的整个DNA序列共观察到6 bp的颠换和4 bp的缺失。为了确定推定的cat-TC基因的活性，对包含来自pTC82的cat决定簇的重组质粒pUC8217进行maxicell分析。基于电泳迁移率，观察到的合成蛋白分子量与从DNA序列预测的27.3 kDa合理吻合。这是第一个报道的罗伊氏乳杆菌Cm抗性决定簇的核苷酸序列，也是将乳酸杆菌添加到在微生物生态系统中自然获得cat-pC194基因的通用细菌属列表中的第一个证据。

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