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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Molecular analysis of two rolling-circle replicating cryptic plasmids, pBMYdx and pBMY1, from the soil gram-positive Bacillus mycoides.
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Molecular analysis of two rolling-circle replicating cryptic plasmids, pBMYdx and pBMY1, from the soil gram-positive Bacillus mycoides.

机译:来自土壤革兰氏阳性芽孢杆菌的两个滚环复制密码质粒pBMYdx和pBMY1的分子分析。

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摘要

Two cryptic plasmids of two environmental strains of the soil Bacillus mycoides were cloned and sequenced. They are of a small size (3377 and 3476 bp) and carry regions homologous to double- and single-strand origins of replication of rolling-circle replication modules. In addition, both plasmids have ORFs with homologies with Mob and Rep proteins, in the same relative position and orientation. While dso- and sso-like sequences are similar in pBMY1 and pBMYdx, the putative Mob and Rep proteins are not homologous between the two but show similarity with Mob and Rep proteins of different bacterial plasmids. Copyright 2000 Academic Press.
机译:克隆了土壤杆菌的两个环境菌株的两个隐性质粒并进行了测序。它们的体积小(3377和3476 bp),携带的区域与滚环复制模块的复制的双链和单链同源。此外,两个质粒在相同的相对位置和方向上均具有与Mob和Rep蛋白同源的ORF。虽然pBMY1和pBMYdx中的dso和sso样序列相似,但假定的Mob和Rep蛋白在两者之间不是同源的,但与不同细菌质粒的Mob和Rep蛋白相似。版权所有2000学术出版社。

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