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首页> 外文期刊>Chemical Reviews >Interfacial Enzymology: The Secreted Phospholipase A_2-Paradigm
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Interfacial Enzymology: The Secreted Phospholipase A_2-Paradigm

机译：界面酶学：磷脂酶A_2-Paradigm

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摘要

Tissue and cellular milieux have microscopically fragmented aqueous compartments separated by vesiculated membranes. Rougly one-half of the protein in cells is estimated to be membrane associated. Many of the membrane enzymes are operationally interfacial enzymes that access the substrate directly from the lipid-water interface.~(16) On the other hand, the noninterfacial membrane-assoicated enzymes,~(7,8) which for brevity we call matrix enzymes, access their substrate directly from the aqueous phase. In the functional sense the matrix enzymes are like the classical soluble enzymes that also access the substrate from the aqueous phase. The observed kinetics of soluble enzymes may be influenced if the enzyme or substrate is in partitioning equilibrium with extraneous interface. In addition, the interface may also have an allosteric effect on the intrinsic catalytic parameters of matrix enzymes as well as on interfacial enzymes.
机译：组织和细胞环境具有微观片段化的水室，被水泡膜隔开。据估计，细胞中一半的蛋白质与膜相关。许多膜酶是可操作的界面酶，可直接从脂质-水界面进入底物。〜（16）另一方面，非界面膜相关的酶〜（7,8）为简洁起见，我们称为基质酶，直接从水相中接近其底物。从功能上讲，基质酶就像经典的可溶性酶一样，它们也可以从水相中进入底物。如果酶或底物与外部界面处于分配平衡状态，则可能会影响观察到的可溶性酶动力学。此外，界面还可能对基质酶的固有催化参数以及界面酶具有变构作用。

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