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首页> 外文期刊>Preparative biochemistry & biotechnology: An international journal for rapid communication >Streptomyces sp TEM 33 possesses high lipolytic activity in solid-state fermentation in comparison with submerged fermentation
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Streptomyces sp TEM 33 possesses high lipolytic activity in solid-state fermentation in comparison with submerged fermentation

机译：与深层发酵相比，链霉菌TEM 33在固态发酵中具有较高的脂解活性

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摘要

Solid-state fermentation (SSF) is a bioprocess that doesn't need an excess of free water, and it offers potential benefits for microbial cultivation for bioprocesses and product development. In comparing the antibiotic production, few detailed reports could be found with lipolytic enzyme production by Streptomycetes in SSF. Taking this knowledge into consideration, we prefer to purify Actinomycetes species as a new source for lipase production. The lipase-producing strain Streptomyces sp. TEM 33 was isolated from soil and lipase production was managed by solid-state fermentation (SSF) in comparison with submerged fermentation (SmF). Bioprocess-affecting factors like initial moisture content, incubation time, and various carbon and nitrogen additives and the other enzymes secreted into the media were optimized. Lipase activity was measured as 1.74 +/- 0.0005 U/g dry substrate (gds) by the p-nitrophenylpalmitate (pNPP) method on day 6 of fermentation with 71.43% final substrate moisture content. In order to understand the metabolic priority in SSF, cellulase and xylanase activity of Streptomyces sp. TEM33 was also measured. The microorganism degrades the wheat bran to its usable form by excreting cellulases and xylanases; then it secretes the lipase that is necessary for degrading the oil in the medium.
机译：固态发酵（SSF）是一种生物过程，不需要过量的游离水，它为生物过程和产品开发中的微生物培养提供了潜在的好处。在比较抗生素产生时，很少有关于链霉菌在SSF中产生脂解酶的详细报道。考虑到这些知识，我们倾向于纯化放线菌作为脂肪酶生产的新来源。产脂肪酶的菌株链霉菌。 TEM 33是从土壤中分离出来的，与固态发酵（SmF）相比，固态发酵（SSF）可以控制脂肪酶的生产。优化了诸如初始水分含量，孵育时间，各种碳和氮添加剂以及分泌到培养基中的其他酶等生物过程影响因素。在发酵的第6天，通过对硝基苯基棕榈酸酯（pNPP）方法将脂肪酶活性测量为1.74 +/- 0.0005U / g干底物（gds），最终底物含水量为71.43％。为了了解在SSF中的代谢优先级，链霉菌属的纤维素酶和木聚糖酶活性。还测量了TEM33。微生物通过分泌纤维素酶和木聚糖酶将麦麸降解为可用形式。然后它会分泌降解培养基中油所需的脂肪酶。

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