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首页> 外文期刊>Platelets >Platelet serotonin transporter (5HTt): physiological influences on kinetic characteristics in a large human population.
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Platelet serotonin transporter (5HTt): physiological influences on kinetic characteristics in a large human population.

机译：血小板血清素转运蛋白（5HTt）：对大量人口的动力学特征的生理影响。

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The present study had two goals: first, to give a detailed description of a reliable method for full kinetic analysis of serotonin transporter (5HTt) on the membrane of human platelets, and second, as a main issue, to report on physiological influences on kinetic characteristics of this transmembrane transport on a large population of healthy individuals. Full kinetic analyses of platelet serotonin uptake were performed on 334 blood donors of both sexes by the use of 14C-radioisotopic method, which was first optimized according to assumptions of enzyme kinetic analyses, with regard to platelet concentration, duration of uptake, concentration of substrate as well as important technical parameters (underpressure of filtration, blanks, incubating temperature, etc). Kinetic parameters of platelet serotonin uptake in the whole population were for V(max): 142 +/- 25.3 pmol 5HT/10(8) platelets/minute and for K(m): 0.404 +/- 0.089 microM 5HT. Besides the report on kinetic values of 5HT transporter protein, we have also described major physiological influences on the mentioned parameters, V(max), K(m) and their derivative, V(max)/K(m) (transporter efficiency): range and frequency distribution of normal values, intraindividual stability over time, lack of age influence, gender dependence and seasonal variations. The report on kinetic values and main physiological influences on platelet serotonin transport kinetics, obtained by the use of thoroughly reassessed methodology, and on by far the largest human population studied until now, offers a reliable frame of reference for pathophysiological studies of this parameter in various clinical fields.
机译：本研究有两个目标：第一，详细描述一种可靠的方法，用于对人体血小板膜上的5-羟色胺转运蛋白（5HTt）进行完整的动力学分析;第二，作为主要问题，报告对动力学的生理影响跨大量健康个体的跨膜转运的特征。通过使用14C放射性同位素方法对334名男女献血者进行了血小板5-羟色胺摄取的全动力学分析，该方法首先根据酶动力学分析的假设进行了优化，涉及血小板浓度，摄取时间，底物浓度以及重要的技术参数（过滤负压，空白，孵育温度等）。整个人群中血小板血清素摄取的动力学参数为V（max）：142 +/- 25.3 pmol 5HT / 10（8）血小板/分钟，K（m）：0.404 +/- 0.089 microM 5HT。除了有关5HT转运蛋白动力学值的报告外，我们还描述了对上述参数V（max），K（m）及其导数V（max）/ K（m）（转运效率）的主要生理影响：正常值的范围和频率分布，个人随时间推移的稳定性，缺乏年龄影响，性别依赖性和季节性变化。该动力学值和对血小板5-羟色胺转运动力学的主要生理影响的报告，是通过彻底重新评估的方法获得的，并且是迄今为止为止研究的最大的人群，为该参数在各种病理生理研究中提供了可靠的参考框架临床领域。

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