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Leukocyte-platelet aggregates-a phenotypic characterization of different stages of peripheral arterial disease

机译：白细胞-血小板聚集体-外周动脉疾病不同阶段的表型特征

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The formation of monocyte-platelet aggregates and neutrophil-platelet aggregates (MPA and NPA, respectively) is influenced by inflammation, but also might contribute to an exacerbation of inflammatory responses in atherosclerotic plaque. The purpose of this study was to analyze MPA and NPA proportions in regard to different stages of peripheral arterial disease (PAD). Forty-five patients with intermittent claudication (IC) (3 groups: Rutherford (R)-1, R-2, and R-3; each n = 15), 20 patients with critical limb ischemia (CLI) (Rutherford 5 (40%) and 6 (60%)), and 20 healthy controls were studied. Analyses of monocyte (Mon) subpopulations (CD14++CD16- (classical) Mon1, CD14++CD16+ (intermediate) Mon2, CD14+CD16++ (non-classical) Mon3), MPA, and NPA was performed from whole blood by flow cytometry. Controls showed an increased proportion of the Mon1 subpopulation (p < 0.001), whereas CLI patients showed a significant increase of the Mon2 subpopulation compared to controls, R-1, or R-2 patients (p < 0.0001). For the Mon3 subpopulation, CLI and R-3 patients showed an increased proportion (p < 0.05). MPA formation with the proinflammatory Mon2 and Mon3 subpopulations was increased in CLI patients (both p < 0.01). Similarly, NPA was significantly increased in CLI patients (p < 0.05). Serological markers of inflammation and procoagulation (fibrinogen [r = 0.459, p < 0.001], soluble triggering receptor expressed on myeloid cells (sTREM-1) [r = 0.237, p < 0.05] and P-Selectin [r = 0.225, p < 0.05]) correlated directly with MPA formation on the Mon2 subpopulation.
机译：单核细胞-血小板聚集体和中性粒细胞-血小板聚集体（分别为MPA和NPA）的形成受炎症影响，但也可能加剧动脉粥样硬化斑块中的炎症反应。本研究的目的是分析外周动脉疾病（PAD）不同阶段的MPA和NPA比例。四十五例间歇性lau行（IC）患者（3组：卢瑟福（R）-1，R-2和R-3;每组n = 15），20例严重肢体缺血（CLI）患者（卢瑟福5（40 ％）和6（60％）），以及20名健康对照者进行了研究。通过流式细胞术从全血中进行单核细胞（Mon）亚群（CD14 ++ CD16-（经典）Mon1，CD14 ++ CD16 +（中级）Mon2，CD14 + CD16 ++（非经典）Mon3），MPA和NPA的分析。对照组显示Mon1亚群的比例增加（p <0.001），而CLI患者显示Mon2亚群与对照组，R-1或R-2患者相比显着增加（p <0.0001）。对于Mon3亚群，CLI和R-3患者的比例有所增加（p <0.05）。在CLI患者中，具有促炎性Mon2和Mon3亚群的MPA形成增加（均p <0.01）。同样，CLI患者的NPA显着增加（p <0.05）。炎症和促凝的血清学标志物（纤维蛋白原[r = 0.459，p <0.001]，在髓样细胞上表达的可溶性触发受体（sTREM-1）[r = 0.237，p <0.05]和P-选择素[r = 0.225，p < 0.05]）与Mon2亚群上MPA的形成直接相关。

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