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首页> 外文期刊>Proteomics >Outer membrane vesicles of the VA-MENGOC-BC (R) vaccine against serogroup B of Neisseria meningitidis: Analysis of protein components by two-dimensional gel electrophoresis and mass spectrometry
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Outer membrane vesicles of the VA-MENGOC-BC (R) vaccine against serogroup B of Neisseria meningitidis: Analysis of protein components by two-dimensional gel electrophoresis and mass spectrometry

机译：针对脑膜炎奈瑟氏球菌B血清群的VA-MENGOC-BC（R）疫苗的外膜囊泡：通过二维凝胶电泳和质谱分析蛋白质成分

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摘要

Neisseria meningitidis is a Gram-negative bacterium responsible for significant mortality worldwide. While effective polysaccharides-based vaccines exist against serogroups A, C, W135, and Y, no similar vaccine is suitable for children under 4 years against disease caused by serogroup B strains. Therefore, major vaccine efforts against this serogroup are based on outer membrane vesicles (OMVs), containing major outer membrane proteins. The OMV-based vaccine produced by the Finlay Institute in Cuba (VA-MENGOC-BC (R)) contributed to the rapid decline of the epidemic in this Caribbean island. While the content of major proteins in this vaccine has been discussed, no detailed work of an outer membrane proteomic map of this, or any other, commercially available OMV-derived product has been published so far. Since OMVs exhibit a large bias toward a few major proteins and usually contain a high content of lipids, establishing the adequate conditions for high resolution, 2-DE of this kind of preparation was definitely a technical challenge. In this work, 2-DE and MS have been used to generate a proteomic map of this product, detailing the presence of 31 different proteins, and it allows the identification of new putative protective protein components it contains.
机译：脑膜炎奈瑟氏球菌是革兰氏阴性细菌，可导致全球范围内的重大死亡。虽然存在针对血清群A，C，W135和Y的有效的基于多糖的疫苗，但没有类似的疫苗适合4岁以下的儿童针对血清群B菌株引起的疾病。因此，针对该血清群的主要疫苗工作基于包含主要外膜蛋白的外膜囊泡（OMV）。古巴Finlay研究所生产的基于OMV的疫苗（VA-MENGOC-BC（R））促成了该加勒比岛屿疫情的迅速下降。尽管已经讨论了该疫苗中主要蛋白质的含量，但迄今为止尚未公开该疫苗或任何其他可商购的OMV衍生产品的外膜蛋白质组图的详细工作。由于OMV对几种主要蛋白质表现出很大的偏倚，并且通常包含高含量的脂质，因此为高分辨率建立了适当的条件，这种制备方法的2-DE无疑是一项技术挑战。在这项工作中，使用2-DE和MS生成该产品的蛋白质组图，详细介绍了31种不同蛋白质的存在，并且可以鉴定其中包含的新的假定保护性蛋白质成分。

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