Expression, purification, and characterization of arginine kinase from the sea cucumber Stichopus japonicus

Guo SY.; Guo Z.; Guo Q.; Chen BY.; Wang XC.

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Expression, purification, and characterization of arginine kinase from the sea cucumber Stichopus japonicus

机译：海参刺参中精氨酸激酶的表达，纯化及鉴定

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The arginine kinase gene of sea cucumber Stichopus japonicus was cloned and inserted into the prokaryotic expression plasmid pET-21b. The protein was expressed in a soluble and functional form in Escherichia coli and purified by Blue Sepharose CL-6B, DEAE-32, and Sephadex G-100 chromotography with a final yield of 83mgL(-1) of LB medium, The specific activity, electrophorctic mobility, and isoelectric focusing were all identical with those of arginine kinase that was purified from sea cucumber muscle. The fluorescence emission spectrum of arginine kinase had a maximum fluorescence at a wavelength of 330 nm upon excitation at 295 nm. These results are the first report of this purified protein. (C) 2003 Elsevier Science (USA). All rights reserved. [References: 20]

机译：克隆了刺参刺参的精氨酸激酶基因，并将其插入原核表达质粒pET-21b中。该蛋白质在大肠杆菌中以可溶性和功能性形式表达，并通过Blue Sepharose CL-6B，DEAE-32和Sephadex G-100层析纯化，最终产量为LB培养基83mgL（-1），比活性，电泳迁移率和等电聚焦均与从海参肌肉中纯化的精氨酸激酶相同。精氨酸激酶的荧光发射光谱在295nm激发时在330nm的波长处具有最大荧光。这些结果是该纯化蛋白的首次报道。（C）2003 Elsevier Science（美国）。版权所有。 [参考：20]

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《Protein Expression and Purification》 |2003年第2期|共5页
作者
Guo SY.; Guo Z.; Guo Q.; Chen BY.; Wang XC.;
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正文语种 eng
中图分类蛋白质;
关键词
Sea cucumber stichopus japonicus; Arginine kinase; Gene clone; Expression; Purification; Characterization; Phosphagen kinase; Sequence-analysis; Creatine-kinase; Evolution; Muscle; Cdna; Gene;

机译：日本刺参;精氨酸激酶;基因克隆;表达;纯化;特征化;磷酸氢激酶;序列分析;肌酸激酶;进化;肌肉;Cdna;基因;

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1. Expression, purification, and characterization of arginine kinase from the sea cucumber Stichopus japonicus [J] . Guo SY., Guo Z., Guo Q., Protein Expression and Purification . 2003,第2期

机译：海参刺参中精氨酸激酶的表达，纯化及鉴定
2. Effects of N-terminal deletion mutation on arginine kinase from the sea cucumber Stichopus japonicus [J] . Liu TT, Wang XC International Journal of Biological Macromolecules: Structure, Function and Interactions . 2008,第1期

机译：N-末端缺失突变对刺参刺参精氨酸激酶的影响
3. The roles of C-terminal loop residues of dimeric arginine kinase from sea cucumber Stichopus japonicus in catalysis, specificity and structure [J] . Zhang HW, Zhao TJ, Wang SL, International Journal of Biological Macromolecules: Structure, Function and Interactions . 2006,第3a5期

机译：日本刺参二聚精氨酸激酶C端环残基在催化，特异性和结构上的作用
4. Characterization of proteases from the digestive tract of sea cucumber (Stichopus japonicus): a new kind of high alkaline protease [C] . Xue-yan Fu, Chang-hu Xue, Ben-chun Miao, International Symposium on the Efficient Application and Preservation of Marine Biological Resourecs; 20041029-1102; Qingdao(CN) . 2004

机译：海参（Stichopus japonicus）消化道中蛋白酶的表征：一种新型的高碱性蛋白酶
5. Expression, purification, and characterization of human pyruvate dehydrogenase kinase isoform 4 (PDK4). [D] . Dong, Jianchun. 2001

机译：人丙酮酸脱氢酶激酶同工型4（PDK4）的表达，纯化和表征。
6. Characterization of proteolysis in muscle tissues of sea cucumber Stichopus japonicus [O] . Chen-Chen Zhao, Yang Yang, Hai-Tao Wu, 2016

机译：海参刺参肌肉组织蛋白水解的表征
7. Uptake and excretion of cobalt by sea cucumber Stichopus japonicus and prawn Penaeus japonicus. [O] . Yuzuru SUZUKI, Motokazu NAKAHARA, Ryoichi NAKAMURA, 1982

机译：海参辛普斯粳稻和虾Penaeus japonicus的摄取和排泄。
8. Purification and Characterization of Polynucleotide Phosphorylase from Cucumber [R] . Khan, Z. A., Fraenkel-Conrat, H. 1985

机译：黄瓜多核苷酸磷酸化酶的分离纯化与鉴定

Expression, purification, and characterization of arginine kinase from the sea cucumber Stichopus japonicus

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