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首页> 外文期刊>Protein Expression and Purification >Purification of the extracellular domain of the membrane protein GlialCAM expressed in HEK and CHO cells and comparison of the glycosylation
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Purification of the extracellular domain of the membrane protein GlialCAM expressed in HEK and CHO cells and comparison of the glycosylation

机译：在HEK和CHO细胞中表达的膜蛋白GlialCAM的胞外域的纯化和糖基化的比较

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摘要

Adhesion molecules are essential for a wide range of biological and physiological functions, including cell-cell interactions, cell intern actions with the extracellular matrix, cell migration, proliferation and survival. Defects in cell adhesion have been associated with pathological conditions such as neoplasia, and neurodegenerative diseases. We have identified a new adhesion molecule of the immunoglobulin family, GlialCAM. The same protein was recently published under the name hepaCAM and was suggested to be associated with hepatocellular carcinoma. Here we have expressed and purified the extracellular domain of this molecule in two mammalian expression systems, HEK and CHO cells. A three step purification protocol gave an over 95% pure protein. The extracellular domain of GlialCAM possesses several potential N- and O-glycosylation sites. Glycosylation is one of the most common post-translational modifications of secreted proteins and of the extracellular domains of membrane bound proteins. It can influence both the activity and the stability of the protein. The glycosylation pattern has been shown to depend on the cell type where the protein is expressed. We examined if differences in the glycosylation of this protein could be detected when it was expressed in the two commonly used mammalian expression systems, HEK and CHO. Differences in the glycosylation were detected. (c) 2007 Elsevier Inc. All rights reserved.
机译：粘附分子对于广泛的生物学和生理功能至关重要，包括细胞间的相互作用，细胞与细胞外基质的内在作用，细胞迁移，增殖和存活。细胞粘附的缺陷与病理状况如瘤形成和神经退行性疾病有关。我们已经确定了免疫球蛋白家族的新粘附分子GlialCAM。最近，该蛋白以hepaCAM的名称发布，并被认为与肝细胞癌有关。在这里，我们已经在两个哺乳动物表达系统HEK和CHO细胞中表达和纯化了该分子的胞外域。三步纯化方案给出了超过95％的纯蛋白。 GlialCAM的胞外域拥有几个潜在的N和O糖基化位点。糖基化是分泌蛋白和膜结合蛋白胞外域最常见的翻译后修饰之一。它可以影响蛋白质的活性和稳定性。已经显示糖基化模式取决于表达蛋白质的细胞类型。我们检查了在两种常用的哺乳动物表达系统HEK和CHO中表达该蛋白时，是否可以检测到该蛋白糖基化的差异。检测到糖基化的差异。 （c）2007 Elsevier Inc.保留所有权利。

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