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首页> 外文期刊>Progress in brain research >Reinnervation of the pretectum in adult rats by regenerated retinal ganglion cell axons: anatomical and functional studies.
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Reinnervation of the pretectum in adult rats by regenerated retinal ganglion cell axons: anatomical and functional studies.

机译：再生的视网膜神经节细胞轴突对成年大鼠再保护神经的解剖和功能研究。

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We have investigated the specificity of reinnervation and terminal arborization of injured retinal ganglion cell (RGC) axons in the brainstem with the object of studying in a simple situation the degree to which regenerating axons are able to replicate the characteristic patterns of terminal arborization and restore normal function. We have focussed here on the pathway that is responsible for the pupillary light reflex, which is mediated through the olivary pretectal nucleus (OPN). In adult rats, the left optic nerve was transected and a segment of peripheral nerve (PN) graft was used to bridge between the retina and different regions of the ipsilateral brainstem, including the superior colliculus. After 4-13 months, regenerated RGC axons were examined in coronal sections stained for cholera toxin B subunit. RGC axons were found extending into the ipsilateral brainstem for distances of up to 6 mm. Within the pretectum, axons innervated the OPN and the nucleus of the optic tract preferentially, and formed distinctive terminal arbors within each. Within the SC axons extended laterally into the visual layers and formed a different type of arborization. On testing the pupillary light reflex, it was found in best cases to show response amplitudes which were comparable to those recorded from control intact animals. However, unlike normals, the response amplitude tended to diminish with repeated stimulation and also appeared to deteriorate with age, although responses could still be detected in some cases as long as 15 months after grafting. These results indicate that regenerating axons can selectively reinnervate denervated nuclei, where they form typical terminal arborizations, and provide the substrates for restoring functional circuitry.
机译：我们研究了受损的视网膜神经节细胞（RGC）轴突在神经干中的神经支配和终末乔化的特异性，目的是在简单的情况下研究再生轴突能够复制终末乔化的特征模式并恢复正常状态的程度。功能。我们在这里集中于负责瞳孔光反射的途径，该途径是通过橄榄前盖核（OPN）介导的。在成年大鼠中，将左视神经切断，并使用一部分周围神经（PN）移植物在视网膜与同侧脑干的不同区域（包括上丘）之间桥接。 4-13个月后，在冠状切片中检查了再生的RGC轴突是否被霍乱毒素B亚基染色。发现RGC轴突延伸到同侧脑干，最大距离为6 mm。在前盖内，轴突优先支配OPN和视神经核，并在每个轴突内形成独特的终末轴。在SC内，轴突侧向延伸到可视层中，并形成了不同类型的乔木。在测试瞳孔光反射时，发现在最佳情况下显示出的响应幅度与对照完整动物记录的响应幅度相当。但是，与正常人不同，尽管在某些情况下甚至可以在移植后长达15个月的时间内仍能检测到反应，但随着反复刺激，反应幅度趋于减小，并且似乎随着年龄的增长而恶化。这些结果表明，再生轴突可以选择性地使失神经的细胞核重新受神经支配，从而形成典型的末端乔木，并为恢复功能电路提供基板。

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