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Pattern stability under cell culture conditions--a comparative study of patterning methods based on PLL-g-PEG background passivation.

机译:细胞培养条件下的图案稳定性-基于PLL-g-PEG背景钝化的图案形成方法的比较研究。

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摘要

Despite the rapidly increasing number of publications on the fabrication and use of micro-patterns for cell studies, comparatively little is know about the long-term stability of such patterns under cell culture conditions. Here, we report on the long-term stability of cellular patterns created by three different patterning techniques: selective molecular assembly patterning, micro-contact printing and molecular assembly patterning by lift-off. We demonstrate that although all three techniques were combined with the same background passivation chemistry based on assembly of a PEG-graft copolymer, there are considerable differences in the long-term stability between the three different pattern types under cell culture conditions. Our results suggest that these differences are not cell-dependent but are due to different (substrate-dependent) interactions between the patterned substrate, the passivating molecule and the serum containing cellular medium.
机译:尽管关于微图案的制备和用于细胞研究的出版物的数量迅速增加,但是对于这种图案在细胞培养条件下的长期稳定性的了解相对较少。在这里,我们报告由三种不同的图案化技术创建的细胞图案的长期稳定性:选择性分子组装图案,微接触印刷和通过剥离的分子组装图案。我们证明,尽管所有这三种技术都基于组装PEG-接枝共聚物的技术与相同的背景钝化化学方法相结合,但是在细胞培养条件下,三种不同模式类型之间的长期稳定性存在很大差异。我们的结果表明,这些差异不是细胞依赖性的,而是归因于图案化底物,钝化分子和含血清的细胞培养基之间的不同(底物依赖性)相互作用。

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