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首页> 外文期刊>Biomaterials >Three-dimensional culture of human disc cells within agarose or a collagen sponge: assessment of proteoglycan production
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Three-dimensional culture of human disc cells within agarose or a collagen sponge: assessment of proteoglycan production

机译：琼脂糖或胶原海绵中的人椎间盘细胞的三维培养：蛋白聚糖生产的评估

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摘要

The objective of the present study was to assess proteoglycan production by human intervertebral disc cells cultured in vitro in selected cell carriers. Based on previous studies which evaluated disc cells seeded into collagen sponge, collagen gel, agarose, alginate or fibrin gel three-dimensional (3D) cell carriers, collagen sponge and agarose were found to provide superior microenvironments for formation of extracellular matrix (ECM). A standardized test design was used to evaluate ECM formed after 14 days of culture using the 1,9-dimethylmethylene blue (DMB) assay to assess sulfated glycosaminoglycan (S-GAG) production. Although agarose culture showed higher S-GAG levels compared to collagen sponge (2.94 +/- 2.20 (19)mu g/ml S-GAG (mean +/- S.D. (n)) vs. 0.94 +/- 0.77 (22), respectively, p = 0.0003), this is off-set by the significantly lower proliferation rate associated with culture of disc cells in agarose. (c) 2005 Elsevier Ltd. All rights reserved.
机译：本研究的目的是评估在选定细胞载体中体外培养的人椎间盘细胞产生蛋白聚糖的情况。根据以前的评估盘状细胞植入胶原海绵，胶原凝胶，琼脂糖，藻酸盐或纤维蛋白凝胶三维（3D）细胞载体的研究，发现胶原海绵和琼脂糖为形成细胞外基质（ECM）提供了优越的微环境。标准化的测试设计用于评估培养14天后形成的ECM，并使用1,9-二甲基亚甲基蓝（DMB）分析评估硫酸化糖胺聚糖（S-GAG）的产生。尽管与胶原蛋白海绵相比，琼脂糖培养物显示出更高的S-GAG水平（2.94 +/- 2.20（19）μg/ ml S-GAG（平均值+/- SD（n））与0.94 +/- 0.77（22），分别为p = 0.0003），这与琼脂糖中圆盘细胞的培养显着降低的增殖速率抵消了。 （c）2005 Elsevier Ltd.保留所有权利。

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