Determination of Cu, Fe, Mn, Zn and total protein content in wheat and soybean samples after sequential extraction procedure [Determina??o de Cu, Fe, Mn, Zn E do teor de proteína total em amostras de trigo e soja após procedimento de extra??o sequencial]

Bittencourt L.M.; Lana D.A.P.D.; Augusti R.; Costa L.M.; Pimenta A.M.C.; Dos Santos A.V.; Gon?alves A.P.F.

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Determination of Cu, Fe, Mn, Zn and total protein content in wheat and soybean samples after sequential extraction procedure [Determina??o de Cu, Fe, Mn, Zn E do teor de proteína total em amostras de trigo e soja após procedimento de extra??o sequencial]

机译：顺序萃取法测定小麦和大豆样品中铜，铁，锰，锌和总蛋白质的含量[测定后小麦和大豆样品中总蛋白质的铜，铁，锰，锌，锌的测定顺序提取]

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A sequential extraction procedure was applied to wheat and soybean seed samples. The total protein content (determined by two distinct methods: Bradford and bicinchoninic acid-BCA) and distribution of Cu, Fe, Mn and Zn in each fraction was determined. The sequential extraction employed four different solutions: water, 0.5 mol L~(-1) NaCl, ethanol/water (70:30 v v~ (-1)) and 0.5 mol L~(-1) NaOH. For both samples, the highest concentration of metals was observed in those extracts associated with globulin-type proteins using NaCl solution. Regarding protein content, higher levels were obtained using the BCA method.

机译：顺序提取程序应用于小麦和大豆种子样品。确定总蛋白质含量（通过两种不同的方法确定：Bradford和双辛可尼酸-BCA）以及每个级分中Cu，Fe，Mn和Zn的分布。顺序萃取采用四种不同的溶液：水，0.5 mol L〜（-1）NaCl，乙醇/水（70:30 v v〜（-1））和0.5 mol L〜（-1）NaOH。对于这两个样品，使用NaCl溶液在与球蛋白型蛋白质相关的提取物中观察到最高的金属浓度。关于蛋白质含量，使用BCA方法获得了更高的水平。

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来源
《Quimica nova》 |2012年第10期|共5页
作者
Bittencourt L.M.; Lana D.A.P.D.; Augusti R.; Costa L.M.; Pimenta A.M.C.; Dos Santos A.V.; Gon?alves A.P.F.;
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正文语种 por
中图分类化学;
关键词
Metals; Protein; Sequential extraction;

机译：金属;蛋白质;顺序萃取;

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1. Determination of Cu, Fe, Mn, Zn and total protein content in wheat and soybean samples after sequential extraction procedure [Determina??o de Cu, Fe, Mn, Zn E do teor de proteína total em amostras de trigo e soja após procedimento de extra??o sequencial] [J] . Bittencourt L.M., Lana D.A.P.D., Augusti R., Quimica nova . 2012,第10期

机译：顺序萃取法测定小麦和大豆样品中铜，铁，锰，锌和总蛋白质的含量[测定后小麦和大豆样品中总蛋白质的铜，铁，锰，锌，锌的测定顺序提取]
2. Determina??o de Cu, Fe, Mn, Zn e do teor de proteína total em amostras de trigo e soja após procedimento de extra??o sequencial [J] . Adriano M. C. Pimenta, Agenor V. Dos Santos, Ana Paula F. Gon?alves, Química Nova . 2012,第10期

机译：顺序萃取后测定小麦和大豆样品中的铜，铁，锰，锌和总蛋白含量
3. Determination of Cu, Fe, Mn, Zn and total protein content in wheat and soybean samples after sequential extraction procedure [J] . Augusti Rodinei, Bittencourt Leila M., Costa Letícia M., Quimica nova . 2012,第10期

机译：序列提取过程中小麦和大豆样品中Cu，Fe，Mn，Zn和总蛋白质含量的测定
4. Determination of Ratios of Auger Electrons Emission Probabilities and K-L Shell Vacancy Transfer Probability of Cr, Mn, Fe, Co, Ni, Cu and Zn Compounds [C] . Adnan Kucukonder, Onur Kavsut Turkish Physical Society International Physics Congress . 2017

机译：Cr，Mn，Fe，Co，Ni，Cu和Zn化合物的螺旋钻电排放概率和K-L壳空位转移概率的测定
5. Clonage et surproduction de superoxyde dismutases a Mn et a Cu,Zn d'Actinobacillus pleuropneumoniae. [D] . Helie, Marie-Claude. 1997

机译：胸膜肺炎放线杆菌锰和铜，锌超氧化物歧化酶的克隆和过量生产。
6. New Green Determination of Cu Fe Mn and Zn in Beetroot Juices along with Their Chemical Fractionation by Solid-Phase Extraction [O] . Pawel Pohl, Mateusz Pieprz, Anna Dzimitrowicz, 2019

机译：甜菜根汁中铜铁锰和锌的新绿色测定及其固相萃取化学分馏
7. Determinação de Cu, Fe, Mn, Zn e do teor de proteína total em amostras de trigo e soja após procedimento de extração sequencial Determination of Cu, Fe, Mn, Zn and total protein content in wheat and soybean samples after sequential extraction procedure [O] . Leila M. Bittencourt, Diogo A. P. D. Lana, Rodinei Augusti, 2012

机译：顺序萃取后测定小麦和大豆样品中的铜，铁，锰，锌和总蛋白含量顺序萃取后测定小麦和大豆样品中的铜，铁，锰，锌和总蛋白含量

Determination of Cu, Fe, Mn, Zn and total protein content in wheat and soybean samples after sequential extraction procedure [Determina??o de Cu, Fe, Mn, Zn E do teor de proteína total em amostras de trigo e soja após procedimento de extra??o sequencial]

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