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首页> 外文期刊>Ultrasound in Medicine and Biology >Real-time ultrasonic assessment of progressive proteoglycan depletion in articular cartilage.
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Real-time ultrasonic assessment of progressive proteoglycan depletion in articular cartilage.

机译：实时超声评估关节软骨中进行性蛋白聚糖的消耗。

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摘要

The loss of proteoglycan (PG) is regarded as one of the early signs of osteoarthritis (OA), thus observing the progress of PG loss would be useful for the early detection of OA. In this study, high-frequency ultrasound was used to monitor and analyze the trypsin-induced progressive degeneration in articular cartilage. Full thickness cartilage-bone specimens (n = 10) prepared from normal bovine patellae were digested using 0.25% trypsin solution for different periods of time to evaluate the dynamics of the digestion process. The trypsin penetration front was observed in M-mode image, which was acquired using a nominal 50 MHz focused transducer. The transient speed of the digestion process was estimated from the image. The digestion fraction, which represents the ratio of the digestion depth to the total cartilage thickness, was estimated from ultrasound data and histology sections. With ultrasound, the digestion fraction observed in the 10 specimens ranged from 64% to 99% and was correlated to that measured by histology (R(2) > or = 0.63, p < 0.05). It was found that the digestion speed decreased nonlinearly with depth from 0.61 +/- 0.16 microm/s (mean +/- SD) in the superficial zone to 0.04 +/- 0.02 microm/s in a region located at 70% of the cartilage thickness in depth. The relationship between the digestion depth and the exposure duration in trypsin could be described using a third order polynomial function. The full thickness of digested and undigested tissues was also measured using caliper, estimated from ultrasound data and histology sections, and compared. These findings indicate that ultrasound could provide useful information about the trypsin-induced progressive PG depletion in articular cartilage. Therefore, ultrasound represents a useful tool to evaluate the dynamics of models of OA in vitro in cartilage specimens in a research environment and this would ultimately help the in vitro examination of articular cartilage for research related to model of OA from the early stages of tissue degradation.
机译：蛋白聚糖（PG）的丢失被认为是骨关节炎（OA）的早期迹象之一，因此观察PG丢失的进展将有助于OA的早期检测。在这项研究中，高频超声被用来监测和分析胰蛋白酶诱导的关节软骨的进行性变性。使用0.25％的胰蛋白酶溶液消化正常牛prepared骨制成的全厚度软骨骨标本（n = 10）不同时间，以评估消化过程的动力学。在M型图像中观察到了胰蛋白酶渗透的前沿，这是使用标称50 MHz聚焦换能器获得的。从图像中估计消化过程的瞬时速度。消化率代表了消化深度与总软骨厚度之比，是根据超声数据和组织学切片估算得出的。超声检查，在10个标本中观察到的消化率介于64％至99％之间，并且与组织学测定的消化率相关（R（2）>或= 0.63，p <0.05）。发现消化速度随深度的变化而非线性降低，从表层区域的0.61 +/- 0.16 microm / s（平均+/- SD）降低到位于软骨70％的区域的0.04 +/- 0.02 microm / s。深度的厚度。可以使用三阶多项式函数来描述胰蛋白酶中消化深度与暴露持续时间之间的关系。还使用卡尺测量了消化和未消化组织的总厚度，并根据超声数据和组织学切片进行了估算，并进行了比较。这些发现表明超声可以提供有关胰蛋白酶诱导的关节软骨中进行性PG消耗的有用信息。因此，超声是评估研究环境中软骨样品中OA模型体外动力学的有用工具，这最终将有助于从组织退化的早期阶段对关节软骨进行体外检查以研究与OA模型相关的研究。

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