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Near-field scanning optical microscopy using polymethylmethacrylate optical fiber probes

机译:使用聚甲基丙烯酸甲酯光纤探头的近场扫描光学显微镜

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摘要

We report the first use of polymethylmethacrylate (PMMA) optical fiber-made probes for scanning near-field optical microscopy (SNOM). The sharp tips were prepared by chemical etching of the fibers in ethyl acetate, and the probes were prepared by proper gluing of sharpened fibers onto the tuning fork in the conditions of the double resonance (working frequency of a tuning fork coincides with the resonance frequency of dithering of the free-standing part of the fiber) reported earlier for the case of glass fibers. Quality factors of the probes in the range 2000-6000 were obtained, which enables the realization of an excellent topographical resolution including state-of-art imaging of single DNA molecules. Near-field optical performance of the microscope is illustrated by the Photon Scanning Tunneling Microscope images of fluorescent beads with a diameter of 100 nm. The preparation of these plastic fiber probes proved to be easy, needs no hazardous material and/or procedures, and typical lifetime of a probe essentially exceeds that characteristic for the glass fiber probe.
机译:我们报告了聚甲基丙烯酸甲酯(PMMA)光纤制造的探针在扫描近场光学显微镜(SNOM)中的首次使用。尖锐的尖端是通过在乙酸乙酯中化学蚀刻纤维而制备的,探针是通过在双共振条件下将尖锐的纤维适当地粘合到音叉上而制备的(音叉的工作频率与共振频率一致)。对于玻璃纤维的情况,纤维的自立部分的抖动已在前面报道过。获得的探针的质量因子在2000-6000范围内,从而能够实现出色的地形分辨率,包括单个DNA分子的最新成像。显微镜的近场光学性能通过直径为100 nm的荧光珠的光子扫描隧道显微镜图像进行说明。这些塑料纤维探针的制备被证明是容易的,不需要有害的材料和/或程序,并且探针的典型寿命基本上超过了玻璃纤维探针的特性。

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