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首页> 外文期刊>Chemistry: A European journal >Conformationally restricted dipyrromethene boron difluoride (BODIPY) dyes: Highly fluorescent, multicolored probes for cellular imaging
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Conformationally restricted dipyrromethene boron difluoride (BODIPY) dyes: Highly fluorescent, multicolored probes for cellular imaging

机译：构象受限的二吡咯亚甲基二氟化硼（BODIPY）染料：用于细胞成像的高荧光，彩色探针

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摘要

Novel fluorescent, conformationally restricted dipyrromethene boron difluoride (BODIPY) dyes have been prepared by introducing a naphthalenyl group at the meso position of the BODIPY core. These BODIPY dyes exhibit increased fluorescence quantum yields compared with dyes that have a meso-position phenyl group with internal rotation. The absorption and emission wavelengths of such conformationally restricted BODIPY dyes can be easily tuned to the near-IR range by derivatization through a condensation reaction with benzaldehyde derivatives. The two-photon absorption properties of these BODIPY dyes were also investigated and the results show that they exhibit increased two-photon excited fluorescence compared to analogue dyes that contain a phenyl group. The one- and two-photon fluorescence imaging of living cells by using selected BODIPY dyes has been successfully demonstrated.
机译：通过在BODIPY核的内消旋位置引入萘基，已经制备了新型的荧光，构象受限的二吡咯亚甲基二氟化硼（BODIPY）染料。与具有内部旋转的中位苯基的染料相比，这些BODIPY染料具有更高的荧光量子产率。这种构象受限的BODIPY染料的吸收和发射波长可以很容易地通过与苯甲醛衍生物的缩合反应进行衍生化而调节到近红外范围。还研究了这些BODIPY染料的双光子吸收特性，结果表明，与含有苯基的类似染料相比，它们显示出增强的双光子激发荧光。通过使用选定的BODIPY染料，已经成功地证明了活细胞的单光子和双光子荧光成像。

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