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首页> 外文期刊>Chemistry: A European journal >A New Aptameric Biosensor for Cocaine Based on Surface-Enhanced Raman Scattering Spectroscopy
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A New Aptameric Biosensor for Cocaine Based on Surface-Enhanced Raman Scattering Spectroscopy

机译：基于表面增强拉曼散射光谱的新型可卡因生物仿制药传感器

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摘要

The present study reports the proof of principle of a reagentless aptameric sensor based on surface-enhanced Raman scattering (SERS) spectroscopy with "signal-on" architecture using a model target of cocaine. This new aptameric sensor is based on the conformational change of the surface-tethered aptamer on a binding target that draws a certain Raman reporter in close proximity to the SERS substrate, thereby increasing the Raman scattering signal due to the local enhancement effect of SERS. To improve the response performance, the sensor is fabricated from a cocaine-templated mixed self-assembly of a 3'-terminal tetramethylrhodamine (TMR)-labeled DNA aptamer oil a silver colloid film by means of an alkanethiol moiety at the 5' end. This immobilization strategy optimizes the orientation of the aptamer on the surface and facilitates the folding on the binding target. Under optimized assay conditions, one can determine cocaine at a concentration of 1 mu M, which compares favorably with analogous aptameric sensors based on electrochemical and fluorescence techniques. The sensor can be readily regenerated by being washed with a buffer. These results suggest that the SERS-based transducer might create a new dimension for future development of aptameric sensors for sensitive determination in biochemical and biomedical studies.
机译：本研究报告了基于可卡因模型靶标的基于表面增强拉曼散射（SERS）光谱和“信号开启”架构的无试剂适配传感器的原理证明。这种新的适体传感器是基于表面束缚适体在结合靶标上的构象变化，该结合靶标吸引了一定的拉曼报告分子紧贴SERS底物，从而由于SERS的局部增强作用而增加了拉曼散射信号。为了提高响应性能，该传感器是由可卡因模板通过3'-末端四甲基罗丹明（TMR）标记的DNA适体油和银胶体膜通过5'端的烷硫醇部分混合自组装而成。该固定策略优化了适体在表面上的取向并促进了在结合靶上的折叠。在优化的测定条件下，可以测定1 µM的可卡因浓度，与基于电化学和荧光技术的类似适体传感器相比具有优势。通过用缓冲液洗涤，可以容易地再生传感器。这些结果表明，基于SERS的传感器可能为适应生物传感器和生物医学研究中敏感测定的适体传感器的未来发展创造新的维度。

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