• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Boronic Acid Functionalized Core—Satellite Composite Nanoparticles forAdvanced Enrichment of Glycopeptides and Glycoproteins
【24h】

Boronic Acid Functionalized Core—Satellite Composite Nanoparticles forAdvanced Enrichment of Glycopeptides and Glycoproteins

机译：硼酸功能化的核-卫星复合纳米颗粒，可实现糖肽和糖蛋白的高级富集

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A core–satellite-structuredcomposite material has been success-fully synthesized for capturing glycosy-lated peptides or proteins. This novelhybrid material is composed of a silica-coated ferrite "core" and numerous"satellites" of gold nanoparticles withlots of "anchors". The anchor, 3-amino-phenylboronic acid, designed for cap-turing target molecules, is highly specif-ic toward glycosylated species. Thelong organic chains bridging the goldsurface and the anchors could reducethe steric hindrance among the boundmolecules and suppress nonspecificbindings. Due to the excellent structure of the current material, the trap-and-release enrichment of glycosylatedsamples is quite simple, specific, andeffective. Indeed, the composite nano-particles could be used for enrichingglycosylated peptides and proteins withvery low concentrations, and the en-riched samples can be easily separatedfrom bulk solution by a magnet. Byusing this strategy, the recovery of gly-copeptides and glycoproteins after en-richment were found to be 85.9 and71.6% separately, whereas the adsorp-tion capacity of the composite nanopar-ticles was proven to be more than79 mg of glycoproteins per gram of thematerial. Moreover, the new compositenanoparticles were applied to enrichglycosylated proteins from human col-orectal cancer tissues for identificationof N-glycosylation sites. In all, 194unique glycosylation sites mapped to155 different glycoproteins have beenidentified, of which 165 sites (85.1 %)were newly identified.
机译：已经成功地合成了一种核-卫星结构复合材料，用于捕获糖基化的肽或蛋白质。这种新型的混合材料由二氧化硅涂层的铁氧体“核”和金纳米颗粒的许多“卫星”以及许多“锚”组成。设计用于捕获目标分子的锚定物3-氨基-苯基硼酸对糖基化物质具有高度特异性。跨越金表面和锚的长有机链可以减少结合分子之间的空间位阻，并抑制非特异性结合。由于当前材料的优良结构，糖基化样品的捕集和释放富集非常简单，特异和有效。实际上，复合纳米颗粒可以用于富集极低浓度的糖基化肽和蛋白质，并且可以通过磁体轻松地将富集样品与大体积溶液分离。通过这种策略，发现富集后糖肽和糖蛋白的回收率分别为85.9和71.6％，而复合纳米颗粒的吸附能力被证明超过每克79毫克糖蛋白材料的此外，新的复合纳米粒子被应用于从人类结肠直肠癌组织中富集糖基化蛋白，以鉴定N-糖基化位点。总共鉴定出了映射到155种不同糖蛋白的194个独特糖基化位点，其中新鉴定出165个位点（占85.1％）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号