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首页> 外文期刊>Urology >Positive prostate-specific antigen circulating cells detected by reverse transcriptase-polymerase chain reaction does not imply the presence of prostatic micrometastases.
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Positive prostate-specific antigen circulating cells detected by reverse transcriptase-polymerase chain reaction does not imply the presence of prostatic micrometastases.

机译：通过逆转录酶-聚合酶链反应检测到的阳性前列腺特异性抗原循环细胞并不意味着存在前列腺微转移。

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OBJECTIVES: Detection of circulating tumor cells may improve the preoperative local staging of prostate cancers. The aim of this study was to perform enhanced reverse transcriptase-polymerase chain reaction (RT-PCR) of prostate-specific antigen (PSA) mRNA to define the predictive value of PSA-positive circulating cells in a large series of patients. METHODS: The study included 46 patients with Stage T1 to T2 prostate cancer, 94 with benign prostatic hyperplasia (BPH), and 51 (including 9 women) with nonprostatic disease. PSA-positive cells from peripheral blood samples were detected by Southern blot analysis of the RT-PCR products. Original oligonucleotide primers were defined to exclusively detect the three PSA mRNA splices. RESULTS: Circulating PSA-positive cells were observed in 8 (8.5%) of 94 patients with BPH, 10 (22%) of 46 with Stage T1 to T2 prostate cancer, and 9 (17.6%) of 51 with nonprostatic disease. The detection rate of PSA-positive circulating cells was significantly increased in patients with prostate cancer versus patients with BPH (P = 0.03). Among clinically localized prostate cancers with a Gleason score less than 8, a correlation was observed between PSA-positive circulating cells and Stage pT3 cancer (P = 0.038), capsular penetration (P = 0.04), and a positive margin (P = 0.038). The specificity of the assay for Stage pT3 cancer detection was 84.6%, with a positive predictive value of 60%. CONCLUSIONS: Although RT-PCR assay may have a role in preoperative local staging, this study demonstrated the absence of tissue and tumor specificity of PSA-positive circulating cells, accounting for the weak positive predictive value of this technique.
机译：目的：检测循环肿瘤细胞可以改善前列腺癌的术前局部分期。这项研究的目的是进行前列腺特异性抗原（PSA）mRNA的增强逆转录聚合酶链反应（RT-PCR），以定义PSA阳性循环细胞在大量患者中的预测价值。方法：该研究包括46例T1至T2期前列腺癌，94例良性前列腺增生（BPH）和51例（包括9名女性）非前列腺疾病。通过RT-PCR产物的Southern印迹分析检测来自外周血样品的PSA阳性细胞。定义原始寡核苷酸引物以专门检测三个PSA mRNA剪接。结果：94例BPH患者中有8例（8.5％）循环PSA阳性细胞，T1至T2期前列腺癌的46例患者中有10例（22％），非前列腺疾病的51例中有9例（17.6％）。与BPH患者相比，前列腺癌患者PSA阳性循环细胞的检出率显着提高（P = 0.03）。在Gleason评分低于8的临床局限性前列腺癌中，观察到PSA阳性循环细胞与pT3期癌（P = 0.038），荚膜穿透（P = 0.04）和阳性切缘（P = 0.038）之间存在相关性。 。该方法对pT3期癌症检测的特异性为84.6％，阳性预测值为60％。结论：尽管RT-PCR分析可能在术前局部分期中起作用，但这项研究表明PSA阳性循环细胞缺乏组织和肿瘤特异性，这说明该技术的阳性预测价值较弱。

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