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A Versatile Anisometric Metallic Supercrystal with Controllable Orientation on a Chip as a Stable and Reliable Label-Free Biosensor

机译:具有稳定方向的可控无标记生物传感器的芯片上具有可控取向的多功能等距金属超晶。

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摘要

Based on anisometric noble-metal nanocrystals, a universal fabrication protocol for preparing 3D supercrystals with controlled orientation on a chip has been developed. A comparison of the surface-enhanced Raman scattering (SERS) behavior of 3D nanorod supercrystals aligned vertically and parallel to the chip indicates that the SERS-enhancing ability and reproducibility of the former is superior to the latter. The 3D nanorod supercrystals aligned vertically to the chip have been utilized as highly sensitive SERS substrates for the label-free discrimination of Gram-positive and -negative bacteria. Furthermore, to strengthen the stability of the supercrystal substrate for assays of bacteria in biosamples, a coating of the antibiotic vancomycin can dramatically increase adhesion of bacteria on a nanointerface and simultaneously improve the SERS response of bacteria to achieve a layer-by-layer assembled, stable, and reliable biosensor for bacteria.
机译:基于不等距的贵金属纳米晶体,已经开发了一种通用的制备方案,用于在芯片上制备取向受控的3D超晶。垂直和平行于芯片排列的3D纳米棒超晶的表面增强拉曼散射(SERS)行为的比较表明,前者的SERS增强能力和重现性优于后者。垂直于芯片排列的3D纳米棒超晶体已被用作高度敏感的SERS底物,用于无标记的革兰氏阳性菌和阴性菌的鉴别。此外,为了增强用于分析生物样品中细菌的超晶基质的稳定性,抗生素万古霉素涂层可以显着提高细菌在纳米界面上的附着力,同时改善细菌的SERS反应,从而实现逐层组装,稳定,可靠的细菌生物传感器。

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