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首页> 外文期刊>Peritoneal dialysis international: Journal of the International Society for Peritoneal Dialysis >Osmotic agents hamper mesothelial repopulation as seen in the doughnut in vivo model.
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Osmotic agents hamper mesothelial repopulation as seen in the doughnut in vivo model.

机译：如在甜甜圈体内模型中所见，渗透剂阻碍了间皮的繁殖。

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BACKGROUND: The problem of mesothelial cell injury derived from the use of peritoneal dialysis solutions has been explored deeply. Conversely, the eventual detrimental effects upon mesothelial cell regeneration have awaked less investigative efforts than those focused on injury. OBJECTIVE: To evaluate in the in vivo and in situ rat "doughnut" model of mesothelial repopulation, the eventual effect of peritoneal lavage with Hank's Balanced Salt Solution (HBSS) as well as that of 4.25% glucose and 7.5% icodextrin dialysis solutions. EXPERIMENTAL ANIMALS: 100 Sprague-Dawley albino rats were included in the study. Animals were divided into five groups of 20 rats each: group 1: control at zero time; group 2: sham-injected rats; group 3: rats exposed to HBSS; group 4: rats treated with 4.25% glucose peritoneal dialysis solution; group 5: rats injected with 7.5% icodextrin. METHODS: Selective exfoliation of a ring of mesothelium (width 0.8 mm, diameter 4 mm) covering the anterior surface of the liver was performed in 80 animals. The control zero-time group was used to evaluate the normal density distribution of the mesothelial cells forming the monolayer. The other groups were treated by means of daily sham injections or intraperitoneal infusion of each experimental solution for a period of 30 consecutive days. After a recovery period of 15 days, imprints and biopsies from the monolayer covering the exfoliated area were taken and processed for light microscopy. RESULTS: Macroscopic observation of the abdominal cavity at the end of the 15-day recovery period showed that the prevalence of fibrotic adhesions between the peritoneal exfoliated area and the neighboring diaphragm was 10% forthe sham-injected group, 5% for the HBSS-exposed animals, 85% for the rats injected with 4.25% glucose, and 95% for the icodextrin-treated group. Prevalence of fibrous adhesions in sham-injected animals and rats exposed to HBSS were devoid of statistically significant differences. Conversely, comparison of these groups with results observed in animals treated with the osmotic agents was significant, at the p < 0.0039 level. Regarding density distribution of mesothelial cells observed in imprints, there were no significant differences between the control zero-time and the sham-injected group. This parameter was marginally lower (p < 0.05) in the HBSS-treated rats. Imprints were not taken from animals exposed to glucose or icodextrin because a dense layer of connective tissue replaced the exfoliated mesothelial area. CONCLUSIONS: Observations made in this study support the contention that both osmotic agents, 4.25% glucose and 7.5% icodextrin, substantially restrain the normal process of mesothelial cell repopulation and induce repair by means of connective tissue. The underlying mechanism is most likely sustained oxidative stress.
机译：背景：腹膜透析液引起的间皮细胞损伤问题已被深入研究。相反，对间皮细胞再生的最终有害影响要比针对损伤的影响少。目的：评估体内和原位大鼠间皮细胞增殖的“甜甜圈”模型，汉克平衡盐溶液（HBSS）以及4.25％葡萄糖和7.5％艾考糊精透析溶液对腹膜灌洗的最终效果。实验动物：包括100只Sprague-Dawley白化病大鼠。将动物分成五组，每组20只大鼠：第一组：在零时间的对照组;第二组在零时。第2组：假注射大鼠;第3组：暴露于HBSS的大鼠;第4组：用4.25％葡萄糖腹膜透析液治疗的大鼠;第5组：大鼠注射7.5％艾考糊精。方法：对80只动物进行了覆盖肝脏前表面的间皮环（宽度0.8 mm，直径4 mm）的选择性剥脱。零时对照组用于评估形成单层的间皮细胞的正常密度分布。其他组通过每天假注射或腹膜内输注每种实验溶液进行治疗，连续30天。在15天的恢复期之后，从覆盖剥落区域的单层上获得印迹和活组织检查，并进行光学显微镜处理。结果：在第15天恢复期结束时对腹腔进行肉眼观察，假注射组腹膜剥离区和邻近diaphragm肌之间的纤维化粘连发生率为10％，HBSS暴露组为5％对于动物，注射了4.25％葡萄糖的大鼠为85％，对艾考糊精治疗组为95％。假注射的动物和暴露于HBSS的大鼠中纤维粘连的发生率没有统计学上的显着差异。相反，将这些组与在用渗透剂处理的动物中观察到的结果进行比较是很明显的，p <0.0039。关于在印迹中观察到的间皮细胞的密度分布，对照组零时间组和假注射组之间没有显着差异。在HBSS治疗的大鼠中，该参数略低（p <0.05）。没有从暴露于葡萄糖或艾考糊精的动物身上获得印记，因为结缔组织的致密层代替了脱落的间皮区域。结论：本研究的观察结果支持以下观点：两种渗透剂：4.25％的葡萄糖和7.5％的艾考糊精，均能显着抑制正常的间皮细胞繁殖过程，并通过结缔组织诱导修复。潜在的机制很可能是持续的氧化应激。

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