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首页> 外文期刊>Taiwanese journal of obstetrics and gynecology >Cryotop vitrification as compared to conventional slow freezing for human embryos at the cleavage stage: survival and outcomes.
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Cryotop vitrification as compared to conventional slow freezing for human embryos at the cleavage stage: survival and outcomes.

机译：与卵裂期人类胚胎的常规慢速冷冻相比，低温冷冻玻璃化：存活和结果。

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OBJECTIVE: This study was conducted to compare the efficacy of cryotop vitrification of human cleavage-stage embryos to that of conventional slow freezing of these embryos with respect to survival. A second objective was to compare the two cryopreservation techniques with respect to outcomes for a cohort of women. MATERIALS AND METHODS: Cleavage-stage embryos from 102 patients were cryopreserved either by vitrification (57 patients) or by traditional slow freezing (45 patients). After thawing, rates of embryo survival, implantation, and clinical pregnancy were determined. RESULTS: Survival of embryos was significantly higher with the vitrification procedure as compared to traditional slow freezing [287/298 (96.3%) vs. 294/446 (65.9%); p < 0.05). Rates of implantation and clinical pregnancy were also significantly higher using vitrification procedure as compared to the slow freezing procedure (24.3% vs. 7.1% and 35.6% vs. 15.6% respectively, p < 0.05). CONCLUSION: As compared to conventional slow freezing, cryopreservation of human cleavage-stage embryo using vitrification results in higher rates of embryo survival, implantation, and clinical pregnancy. Vitrification therefore represents the superior cryopreservation technique for cleavage-stage embryos.
机译：目的：本研究旨在比较人类卵裂期胚胎低温冷冻玻璃化与常规慢速冷冻这些胚胎在存活方面的功效。第二个目标是比较两种冷冻保存技术在一组妇女的预后方面。材料与方法：通过玻璃化（57例）或传统的慢速冷冻（45例）冷冻保存102例患者的卵裂期胚胎。解冻后，确定胚胎存活率，植入率和临床妊娠率。结果：与传统的慢速冷冻相比，玻璃化过程的胚胎存活率显着更高[287/298（96.3％）与294/446（65.9％）; p ＜0.05）。与缓慢冷冻程序相比，使用玻璃化程序的植入率和临床妊娠率也显着更高（分别为24.3％比7.1％和35.6％比15.6％，p <0.05）。结论：与传统的慢速冷冻相比，使用玻璃化技术冷冻保存人类卵裂期的胚胎可提高胚胎存活率，植入率和临床妊娠率。因此，玻璃化代表了卵裂期胚胎的卓越冷冻保存技术。

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