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首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >2D spatiotemporal visualization system of expired gaseous ethanol after oral administration for real-time illustrated analysis of alcohol metabolism
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2D spatiotemporal visualization system of expired gaseous ethanol after oral administration for real-time illustrated analysis of alcohol metabolism

机译：口服后已过期的气态乙醇的二维时空可视化系统，用于酒精代谢的实时图解分析

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摘要

A novel 2-dimensional spatiotemporal visualization system of expired gaseous ethanol after oral administration for real-time illustrated analysis of alcohol metabolism has been developed, which employed a low level light CCD camera to detect chemiluminescence (CL) generated by catalytic reactions of standard gaseous ethanol and expired gaseous ethanol after oral administration. First, the optimization of the substrates for visualization and the concentration of luminol solution for CL were investigated. The cotton mesh and 5.0 mmol L~(-1) luminol solution were selected for further investigations and this system is useful for 0.1-20.0 mmol L~(-1) of H_2O_2 solution. Then, the effect of pH condition of Tris-HCl buffer solution was also evaluated with CL intensity and under the Tris-HCl buffer solution pH 10.1, a wide calibration range of standard gaseous ethanol (30-400 ppm) was obtained. Finally, expired air of 5 healthy volunteers after oral administration was measured at 15, 30, 45, 60, 75, 90, 105 and 120 min after oral administration, and this system showed a good sensitivity on expired gaseous ethanol for alcohol metabolism. The peaks of expired gaseous ethanol concentration appeared within 30 min after oral administration. During the 30 min after oral administration, the time variation profile based on mean values showed the absorption and distribution function, and the values onward showed the elimination function. The absorption and distribution of expired gaseous ethanol in 5 healthy volunteers following first-order absorption process were faster than the elimination process, which proves efficacious of this system for described alcohol metabolism in healthy volunteers. This system is expected to be used as a non-invasive method to detect VOCs as well as several other drugs [1] in expired air for clinical purpose.
机译：开发了一种新颖的二维时空可视化可视化系统，用于口服给药后的气态乙醇的实时醇解代谢分析，该系统采用低照度CCD摄像头检测标准气态乙醇催化反应产生的化学发光（CL）。并在口服后释放出气态乙醇。首先，研究了用于可视化的基质的优化和用于CL的鲁米诺溶液的浓度。选择棉网和5.0 mmol L〜（-1）鲁米诺溶液进行进一步研究，该系统可用于0.1〜20.0 mmol L〜（-1）的H_2O_2溶液。然后，还用CL强度评估了Tris-HCl缓冲溶液的pH条件的影响，并且在Tris-HCl缓冲溶液的pH 10.1下，获得了宽范围的标准气态乙醇（30-400 ppm）校准范围。最后，在口服后15、30、45、60、75、90、105和120分钟测量了5名健康志愿者在口服后的呼出空气，该系统对呼出的乙醇对酒精代谢具有良好的敏感性。口服后30分钟内，呼出的乙醇气体浓度达到峰值。口服后30分钟内，基于平均值的时间变化曲线显示了吸收和分布函数，而随后的值显示了消除函数。一级吸收过程之后，在5名健康志愿者中，过期气态乙醇的吸收和分布比清除过程要快，这证明了该系统对于健康志愿者中所描述的酒精代谢是有效的。该系统有望作为一种非侵入性方法来检测呼出气中的VOC以及其他几种药物[1]，以用于临床目的。

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