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首页> 外文期刊>Chemistry: A European journal >Pyrene-Stacked Nanostructures Constructed in the Recombinant Tobacco Mosaic Virus Rod Scaffold
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Pyrene-Stacked Nanostructures Constructed in the Recombinant Tobacco Mosaic Virus Rod Scaffold

机译：烟草花叶病毒棒支架中构建的堆叠纳米结构。

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The effect of pyrenes introduced into a tobacco mosaic virus(TMV)coat protein monomer on the formation and stability of the TMV assembly was investigated.The possible arrangement of the pyrenes in the inner cavity of the TMV rod was also estimated.The pyrene derivative was introduced to four specific amino acids in the cavity of the TMV rod structure.Rod-structure formation was examined by atomic force microscopy(AFM).Two pyrene-attached mutants(positions 99 and 100)assembled to increase the length of the rod structures by 2.5 mum at pH 5.5.The interaction of the pyrene moieties in the TMV cavity was investigated by steady-state and time-resolved spectroscopic analysis.Strong excimer emission with significantly short wavelength(465 nm)was observed from the two mutants mentioned above.Excitation and UV-visi-ble spectra indicate that the pyrene moieties form pi-stacked structures in the TMV cavity.Details of the pyrene interaction were investigated by analyzing the fluorescence lifetime of the excimer.Results suggest that the pyrenes formed preassociated rigid structures with partially overlapped geometry in the restricted space of the TMV cavity.The pyrenes effectively stabilize the TMV rod through a it-stacking interaction in a well-ordered way,and the single pyrene moiety introduced into the monomer affects the overall formation of the TMV rod structure.
机译：研究了of引入烟草花叶病毒（TMV）外壳蛋白单体中对on病毒组装体形成和稳定性的影响，还估算了the在in病毒棒内腔中的可能排列方式，was为引入到TMV杆结构腔中的四个特定氨基酸上。通过原子力显微镜（AFM）检查杆结构的形成。组装了两个带有pyr的突变体（位置99和100）以增加杆结构的长度pH值为5.5时为2.5μm。通过稳态和时间分辨光谱分析研究了TMV腔中the部分的相互作用，从上述两个突变体观察到强短波长（465 nm）的强准分子发射。紫外可见光谱表明the部分在TMV腔中形成π堆积结构。通过分析荧光寿命研究了interaction相互作用的细节。结果表明，the在TMV腔的受限空间内形成了预缔合的刚性结构，其几何结构部分重叠..通过有序的IT堆积相互作用和单个pyr有效地稳定了TMV杆。引入单体中的部分会影响TMV棒结构的整体形成。

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