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Twin Probes as a Novel Tool for the Detection of Single-Nucleotide Polymorphisms

机译：双探针作为检测单核苷酸多态性的新型工具

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摘要

Single-nucleotide polymorphisms(SNPs)are the most common form of DNA sequence variation.There is a strong interest from both academy and industry to develop rapid,sensitive and cost effective methods for SNP detection.Here we report a novel structural concept for DNA detection based on fluorescence dequenching upon hybridization.The so-called"twin probe"consists of a central fluorene derivative as fluorophore to which two identical oligonucleotides are covalent-ly attached.This probe architecture is applied in homogeneous hybridization assays with subsequent fluorescence spectroscopic analysis.The bioorganic hybrid structure is well suited for sequence specific DNA detection and even SNPs are identified with high efficiency.Additionally,the photophysical properties of the twin probe were investigated.The covalent attachment of two single stranded oligonucleotides leads to strong quenching of the central fluorescence dye induced by the nu-cleobases.The twin probe is characterized by supramolecular aggregate formation accompanied by red-shifted emission and broad fluorescence spectra.
机译：单核苷酸多态性（SNPs）是最常见的DNA序列变异形式。学术界和工业界都强烈希望开发出快速，灵敏且经济高效的SNP检测方法。在此，我们报道了一种新颖的DNA检测结构概念。所谓的“双探针”由中心芴衍生物作为荧光团组成，其中两个相同的寡核苷酸共价连接。这种探针结构用于均质杂交测定及随后的荧光光谱分析中。生物有机杂化结构非常适合序列特异性DNA检测，甚至可以高效鉴定SNP。此外，还研究了双探针的光物理性质。两个单链寡核苷酸的共价连接导致诱导的中央荧光染料强烈淬灭双核探针的特征是分子聚集体的形成伴随着红移发射和宽广的荧光光谱。

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