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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Genomic sequencing of deer tick virus and phylogeny of powassan-related viruses of North America.
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Genomic sequencing of deer tick virus and phylogeny of powassan-related viruses of North America.

机译:北美鹿壁虱病毒的基因组测序和波瓦桑相关病毒的系统发育。

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摘要

Powassan (POW) virus is responsible for central nervous system infection in humans in North America and the eastern parts of Russia. Recently, a new flavivirus, deer tick (DT) virus, related to POW virus was isolated in the United States, but neither its pathogenic potential in human nor the taxonomic relationship with POW virus has been elucidated. In this study, we obtained the near-full-length genomic sequence of the DT virus and complete sequences of 3 genomic regions of 15 strains of POW-related virus strains. The phylogeny revealed 2 lineages, one of which had the prototype POW virus and the other DT virus. Both lineages can cause central nervous system infection in humans. By use of the combination of molecular definition of virus species within the genus Flavivirus and serological distinction in a 2-way cross-neutralization test, the lineage of DT virus is classified as a distinct genotype of POW virus.
机译:Powassan(POW)病毒是导致北美和俄罗斯东部地区人类中枢神经系统感染的原因。最近,在美国分离了一种新的黄病毒,与POW病毒有关的鹿壁虱(DT)病毒,但尚未阐明其对人的致病性或与POW病毒的分类学关系。在这项研究中,我们获得了DT病毒的近全长基因组序列,以及15个POW相关病毒株的3个基因组区域的完整序列。系统发育揭示了两个谱系,其中一个具有原型POW病毒,另一个具有DT病毒。两种谱系均可导致人类中枢神经系统感染。通过在两向交叉中和试验中结合黄病毒属病毒种类的分子定义和血清学区别,DT病毒谱系被归类为POW病毒的独特基因型。

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