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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >REAL-TIME REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION QUANTIFICATION OF WEST NILE VIRUS TRANSMITTED BY CULEX PIPIENS QUINQUEFASCIATUS.
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REAL-TIME REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION QUANTIFICATION OF WEST NILE VIRUS TRANSMITTED BY CULEX PIPIENS QUINQUEFASCIATUS.

机译：CUPI PIPIENS QUINQUEFASCIATUS传递的西尼勒病毒的实时逆转录酶-聚合酶链反应定量。

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摘要

Transmission experiments are a critical component of vector competence studies. In this study, a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was used to enumerate the amount of West Nile virus (WNV) secreted in mosquito saliva following oral infection. Culex pipiens quinquefasciatus were allowed to feed on WNV-infected blood, and saliva was collected on days 14 and 21 post-infection (pi). The amount of virus at these two time points varied significantly, with mean equivalent plaque-forming units (pfu) of approximately 30,500 on day 14 pi and 5,800 on day 21 pi. Individual mosquitoes secreted up to 2 x 10(5) pfu of virus. Titer of whole mosquitoes and immunofluorescence assay of salivary glands from mosquitoes collected at these two time points were also used for supplemental comparison. This report describes the first use of a real-time RT-PCR to quantify the amount of WNV in mosquito saliva.
机译：传播实验是媒介能力研究的关键组成部分。在这项研究中，实时逆转录聚合酶链反应（RT-PCR）被用于枚举口腔感染后蚊唾液中分泌的西尼罗河病毒（WNV）的数量。允许淡色库蚊（Culex pipiens quinquefasciatus）以WNV感染的血液为食，并在感染后第14天和第21天（pi）收集唾液。在这两个时间点的病毒数量差异很大，在pi第14天的平均等效噬菌斑形成单位（pfu）约为第30天的第30天，而在pi 21的第天的平均等效噬菌斑形成单位（pfu）约为5,800。单个蚊子分泌高达2 x 10（5）pfu的病毒。还使用了整个蚊的滴度和在这两个时间点收集的蚊子唾液腺的免疫荧光测定法进行了补充比较。该报告介绍了实时RT-PCR首次用于定量蚊唾液中WNV的量。

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