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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Identification in triatomine vectors of feeding sources and Trypanosoma cruzi variants by heteroduplex assay and a multiplex miniexon polymerase chain reaction.
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Identification in triatomine vectors of feeding sources and Trypanosoma cruzi variants by heteroduplex assay and a multiplex miniexon polymerase chain reaction.

机译：通过异源双链体分析和多重miniexon聚合酶链反应在饲料来源和克氏锥虫变体的三松碱载体中进行鉴定。

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摘要

Feeding sources of triatomine vectors (Triatoma longipennis) collected in peridomiciles in Mexico were identified by a heteroduplex assay developed with triatomine blood meals. Trypanosoma cruzi parasites were also characterized in the same blood meal samples by multiplex-polymerase chain reaction assay of mini-exon gene inter-genic regions. The main blood meal source was from rats, but the bugs were able to feed on a wide variety of hosts, and human blood meals were identified. Trypanosoma cruzi was the only flagellate species identified in the blood meals. All populations belong to the T. cruzi I lineage, a result that is consistent with the previously assumed predominance of this lineage in Mexico. This combination of blood meal and T. cruzi lineage identification provides a powerful tool for understanding T. cruzi transmission cycles.
机译：在墨西哥围裙动物中收集的三atomine载体（Triatoma longipennis）的饲料来源是通过用三atomine血粉开发的异源双链体分析确定的。克氏锥虫寄生虫还通过微型外显子基因间基因区的多重聚合酶链反应测定法在同一血粉样品中表征。主要的血粉来源是大鼠，但虫子能够以多种宿主为食，并鉴定出人类的血粉。克氏锥虫是血粉中唯一鉴定出的鞭毛物种。所有种群均属于克鲁斯氏菌I谱系，这一结果与该谱系先前在墨西哥占主导地位的事实相符。血粉和克氏锥虫谱系鉴定的这种结合为理解克氏锥虫传播周期提供了强大的工具。

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