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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Molecular identification of blood-meal sources in Culiseta melanura and Culiseta morsitans from an endemic focus of eastern equine encephalitis virus in New York.
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Molecular identification of blood-meal sources in Culiseta melanura and Culiseta morsitans from an endemic focus of eastern equine encephalitis virus in New York.

机译：从纽约东部马脑炎病毒的流行焦点出发，对黑腹Culiseta meluraura和Culiseta morsitans中的血粉来源进行分子鉴定。

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摘要

Eastern equine encephalitis (EEE) virus perpetuates in an enzootic cycle involving ornithophilic mosquito vectors, principally Culiseta melanura (Coquillett) and avian amplification hosts. To better understand the role of Cs. melanura and Culiseta morsitans (Theobald) in the epizootiology of EEE virus, we collected blood-fed mosquitoes between 31 May and 15 October 2004 at two sites associated with an EEE virus focus in central New York and identified the source of vertebrate blood by nucleotide sequencing of polymerase chain reaction (PCR) products of the cytochrome b gene. Analysis of 484 Cs. melanura and 122 Cs. morsitans revealed that 94.2% and 86.9%, respectively, acquired blood solely from avian hosts. Blood meals derived exclusively from mammals were detected in 0.8% of Cs. melanura and 1.6% of Cs. morsitans. Individual mosquitoes containing mixed-blood meals from both avian and mammalian hosts were also detected in 5.0% of Cs. melanura and 11.5% of Cs. morsitans. Wood thrush constituted the most common vertebrate host for Cs. melanura (23.6%) and Cs. morsitans (30.9%), followed by American robin, song sparrow, ovenbird, red-eyed vireo, and common yellowthroat. Mammalian-derived blood meals were identified as white-tailed deer, horse, domestic cat, and eastern pipistrelle bat. There were three isolations of EEE virus from Cs. melanura and one from Cs. morsitans. These results suggest that wood thrush and a few other passerine birds may play key roles in supporting EEE virus transmission in the northeast and possibly throughout the geographic range of EEE in North America. The frequency of mammalian feedings also suggests that Cs. melanura and Cs. morsitans may play a role in the transmission of EEE virus to equines, in addition to maintaining enzootic transmission among avian hosts. We report the first isolation of arboviruses from mosquito vectors concomitant with the identifications of their blood meal sources.
机译：东部马脑炎（EEE）病毒在涉及嗜鸟性蚊媒（主要是黑腹锦鸡（Coquillett）和禽类扩增宿主）的致盲周期中持续存在。为了更好地了解Cs的作用。我们在2004年5月31日至10月15日之间，在纽约市中心的两个与EEE病毒有关的地点收集了食血蚊子，并通过核苷酸测序鉴定了脊椎动物血液的来源细胞色素b基因的聚合酶链反应（PCR）产物的鉴定。分析484 Cs。 melanura和122 Cs。 morsitans透露，分别有94.2％和86.9％的血液仅来自禽类宿主。仅在0.8％的Cs中检测到仅来自哺乳动物的血粉。黑色素和Cs的1.6％。 morsitans。在5.0％的Cs中也检测到含有来自禽类和哺乳动物宿主的混合血粉的单个蚊子。黑色素和Cs的11.5％。莫西坦人。鹅口疮是Cs最常见的脊椎动物宿主。黑色素（23.6％）和Cs。 morsitans（30.9％），其次是美国知更鸟，麻雀，火鸟，红眼的vireo和常见的黄喉。哺乳动物来源的血粉被鉴定为白尾鹿，马，家猫和东方。从Cs中分离出了三种EEE病毒。黑色和一个来自CS。 morsitans。这些结果表明，鹅口疮和其他一些雀形目鸟类可能在支持东北和整个北美EEE地理范围的EEE病毒传播中起关键作用。哺乳的频率也暗示了Cs。 melanura和CS。除维持禽类宿主之间的动物传播外，morsitans还可能在EEE病毒向马的传播中起作用。我们报告首次从蚊媒中分离出虫媒病毒，并伴随其血粉来源的鉴定。

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