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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Growth characteristics of ChimeriVax-Den vaccine viruses in Aedes aegypti and Aedes albopictus from Thailand.
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Growth characteristics of ChimeriVax-Den vaccine viruses in Aedes aegypti and Aedes albopictus from Thailand.

机译：ChimeriVax-Den疫苗病毒在泰国伊蚊和白纹伊蚊中的生长特征。

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摘要

Four chimeric yellow fever (YF) 17D-dengue (DEN) candidate vaccine viruses (ChimeriVax-DEN; Acambis, Cambridge, MA) were characterized in Aedes aegypti and Ae. albopictus mosquitoes collected from Thailand. The four vaccine viruses contained the relevant prM and E genes of wild-type dengue viruses (DENV; serotypes 1-4) substituted for the equivalent genes in the YF vaccine virus (17D) backbone. Each chimera conferred protection against the homologous DENV serotype; a tetravalent mix of all four chimeras stimulates an immune response against all serotypes. Field-collected mosquitoes from Thailand were fed on blood containing each of the viruses under study and held 21 days after infection. Infection and dissemination rates were based on antigen detection in the body or head tissues, respectively. All four wild-type DENV serotypes infected and disseminated, but the candidate vaccine viruses were highly attenuated in mosquitoes with respect to infection and especially with respect to dissemination. Considering the low level viremias anticipated in humans vaccinated with these viruses, it is predicted that the risks of infection and transmission by mosquitoes in nature is minimal.
机译：在埃及伊蚊和埃及伊蚊中鉴定了四种嵌合黄热病（YF）17D-登革热（DEN）候选疫苗病毒（ChimeriVax-DEN; Acambis，剑桥，马萨诸塞州）。从泰国收集的白化蚊。四种疫苗病毒包含野生型登革热病毒（DENV;血清型1-4）的相关prM和E基因，以取代YF疫苗病毒（17D）骨架中的等效基因。每个嵌合体均具有针对同源DENV血清型的保护作用。所有四个嵌合体的四价混合物刺激针对所有血清型的免疫应答。将来自泰国的现场采集的蚊子喂食含有每种正在研究的病毒的血液，并在感染后21天关押。感染率和传播率分别基于人体或头部组织中的抗原检测。所有四种野生型DENV血清型均被感染和传播，但是在感染方面，特别是在传播方面，候选疫苗病毒在蚊子中高度减毒。考虑到在用这些病毒接种的人类中预期的低水平病毒血症，预计自然界中蚊子感染和传播的风险极小。

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