Discrimination of Seven Anopheles Species from San Pedro de Uraba, Antioquia, Colombia, by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Its Sequences.

Zapata MA; Cienfuegos AV; Quiros OI; Quinones ML; Luckhart S; Correa MM

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Discrimination of Seven Anopheles Species from San Pedro de Uraba, Antioquia, Colombia, by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Its Sequences.

机译：通过序列的聚合酶链反应-限制性片段长度多态性分析，区分了哥伦比亚安蒂奥基亚省的圣佩德罗-德乌拉巴的七个按蚊物种。

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Accurate identification of anopheline species is essential for vector incrimination and implementation of appropriate control strategies. Several anopheline species are considered important malaria vectors in Colombia; however, species determination is complicated by cryptic morphology and intra-individual variation. We describe polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of internal transcribed spacer 2 (ITS2) sequences for differentiation of seven Anopheles species collected in a locality in Antioquia, Colombia, with high levels of malaria transmission. Each of these seven species can be identified by unique AluI PCR-RFLP restriction patterns. Comparisons of morphologic identification with molecular identification of voucher specimens confirmed species designation for 886 wild-caught anophelines. This new method can be used as a diagnostic tool for discrimination of anopheline species of medical importance in this region, some of which have overlapping morphologic characters and for conducting complementary studies where rapid and accurate identification of large numbers of specimens is needed.

机译：准确鉴定按蚊种对于媒介鉴定和实施适当控制策略至关重要。在哥伦比亚，几种按蚊属被认为是重要的疟疾媒介。然而，物种的确定由于隐秘的形态和个体内部的变异而变得复杂。我们描述了内部转录间隔区2（ITS2）序列的聚合酶链反应-限制性片段长度多态性（PCR-RFLP），用于区分在哥伦比亚Antioquia，高水平疟疾传播地区收集的七个按蚊物种。这七个物种中的每一个都可以通过独特的AluI PCR-RFLP限制性酶切模式进行鉴定。凭证标本的形态学鉴定与分子鉴定的比较证实了886种野生捕获的按蚊的物种名称。这种新方法可以用作诊断该地区医学上重要的按蚊种的诊断工具，其中一些具有重叠的形态特征，并且可以用于需要快速而准确地鉴定大量标本的补充研究。

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来源
《The American Journal of Tropical Medicine and Hygiene》 |2007年第1期|共6页
作者
Zapata MA; Cienfuegos AV; Quiros OI; Quinones ML; Luckhart S; Correa MM;
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正文语种 eng
中图分类地方病学;
关键词
Polymorphism; Restriction Fragment Length; Polymerase Chain Reaction; anophelin; 多态性; 限制性片段长度; 聚合酶链反应; 哥伦比亚;

机译：Polymorphism;Restriction Fragment Length;Polymerase Chain Reaction;anophelin;多态性;限制性片段长度;聚合酶链反应;哥伦比亚;

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1. Discrimination of Seven Anopheles Species from San Pedro de Uraba, Antioquia, Colombia, by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Its Sequences. [J] . Zapata MA, Cienfuegos AV, Quiros OI, The American Journal of Tropical Medicine and Hygiene . 2007,第1期

机译：通过序列的聚合酶链反应-限制性片段长度多态性分析，区分了哥伦比亚安蒂奥基亚省的圣佩德罗-德乌拉巴的七个按蚊物种。
2. Identification of shrimp species in raw and processed food products by means of a polymerase chain reaction-restriction fragment length polymorphism method targeted to cytochrome b mitochondrial sequences. [J] . Pascoal A, Barros-Velazquez J, Cepeda A, Electrophoresis: The Official Journal of the International Electrophoresis Society . 2008,第15期

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3. Rapid polymerase chain reaction-restriction fragment length polymorphism method for discrimination of the two Atlantic cryptic deep-sea species of scabbardfish [J] . Stefanni Sergio, Bettencourt Raul, Knutsen Halvor, Molecular ecology resources . 2009,第2期

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6. Comparison of CHROMagar polymerase chain reaction-restriction fragment length polymorphism and polymerase chain reaction-fragment size for the identification of Candida species [O] . Zahra Jafari, Marjan Motamedi, Nilufar Jalalizand, 2017

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8. Population Genetics Study of Anopheles Darlingi (Diptera: Culicidae) from Colombia Based on Random Amplified Polymorphic DNA-Polymerase Chain Reaction and Amplified Fragment Length Polymorphism Markers [R] . Gonzalez, R., Wilkerson, R., Suarez, M. F., 2007

机译：基于随机扩增多态性DNa聚合酶链反应和扩增片段长度多态性标记的哥伦比亚按蚊Darleri（双翅目：蚊科）的群体遗传学研究

Discrimination of Seven Anopheles Species from San Pedro de Uraba, Antioquia, Colombia, by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Its Sequences.

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