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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Detection and stability of Japanese encephalitis virus RNA and virus viability in dead infected mosquitoes under different storage conditions.
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Detection and stability of Japanese encephalitis virus RNA and virus viability in dead infected mosquitoes under different storage conditions.

机译：在不同储存条件下死者感染的蚊子中日本脑炎病毒RNA和病毒生存力的检测和稳定性。

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摘要

A semi-nested polymerase chain reaction (PCR) was evaluated for detection of Japanese encephalitis (JE) virus in infected mosquitoes stored under simulated northern Australian summer conditions. The effect of silica gel, thymol, and a combination of the two on RNA stability and virus viability in dead mosquitoes were also examined. While JE virus RNA was relatively stable in mosquitoes held for up to 14 days after death, viable virus was not detected after day 1. Thymol vapor inhibited fungal contamination. Detection of single mosquitoes infected with JE virus in large pools of mosquitoes was also investigated. Single laboratory-infected mosquitoes were detected in pools of < or = 200 mosquitoes and in pools diluted to 0.2/100 and 0.1/100 mosquitoes, using the semi-nested PCR. However, the ability to detect live virus decreased as pool size increased. The semi-nested PCR proved more expensive than virus isolation for pools of 100 mosquitoes. However, the semi-nested PCR was faster and more economical using larger pools. Results indicate that surveillance of JE virus in mosquitoes using the semi-nested PCR is an alternative to monitoring seroconversions in sentinel pigs.
机译：评估了半巢式聚合酶链反应（PCR），以检测在模拟北澳大利亚夏季条件下储存的感染蚊子中的日本脑炎（JE）病毒。还检查了硅胶，百里香酚以及两者的组合对死蚊中RNA稳定性和病毒生存力的影响。虽然JE病毒RNA在死亡后长达14天的蚊子中相对稳定，但在第1天后仍未检测到活病毒。百里酚蒸气可抑制真菌污染。还研究了在大量蚊子中检测到感染了JE病毒的单个蚊子的情况。使用半巢式PCR，在小于或等于200的蚊子池中以及稀释到0.2 / 100和0.1 / 100蚊子的池中检测到单个实验室感染的蚊子。但是，随着池大小的增加，检测活病毒的能力也会降低。半巢式PCR证明比100个蚊子的蚊子隔离更昂贵。但是，使用较大的池时，半嵌套式PCR更快，更经济。结果表明，使用半巢式PCR监测蚊子中的JE病毒是监测前哨猪血清转化的一种替代方法。

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