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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Preclinical Vaccine Study of Plasmodium vivax Circumsporozoite Protein Derived-Synthetic Polypeptides Formulated in Montanide ISA 720 and Montanide ISA 51 Adjuvants
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Preclinical Vaccine Study of Plasmodium vivax Circumsporozoite Protein Derived-Synthetic Polypeptides Formulated in Montanide ISA 720 and Montanide ISA 51 Adjuvants

机译：间日疟原虫环子孢子蛋白衍生的合成多肽在Montanide ISA 720和Montanide ISA 51佐剂中的临床前疫苗研究

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摘要

Plasmodium vivax circumsporozoite (CS) protein is a leading malaria vaccine candidate previously assessed in animals and humans. Here, combinations of three synthetic polypeptides corresponding to amino (N), central repeat (R), and carboxyl (C) regions of the CS protein formulated in Montanide ISA 720 or Montanide ISA 51 adjuvants were assessed for immunogenicity in rodents and primates. BALB/c mice and Aotus monkeys were divided into test and control groups and were immunized three times with doses of 50 and 100 mug of vaccine or placebo. Antigen-specific antima-larial antibodies were determined by enzyme-linked immunosorbent assay, immunofluorescent antibody test, and IFN-gamma responses by enzyme-linked immunosorbent spot (ELIspot). Both vaccine formulations were highly immunogenic in both species. Mice developed better antibody responses against C and R polypeptides, whereas the N polypeptide was more immunogenic in monkeys. Anti-peptide antibodies remained detectable for several months and recognized native proteins on sporozoites. Differences between Montanide ISA 720 and Montanide ISA 51 formulations were not significant.
机译：间日疟原虫环子孢子（CS）蛋白是先前在动物和人类中评估过的领先的疟疾疫苗候选者。在此，评估了在Montanide ISA 720或Montanide ISA 51佐剂中配制的CS蛋白的氨基（N），中心重复（R）和羧基（C）区域的三种合成多肽的组合在啮齿动物和灵长类动物中的免疫原性。将BALB / c小鼠和Aotus猴子分为测试组和对照组，并分别用50和100杯疫苗或安慰剂免疫3次。抗原特异性抗疟疾抗体通过酶联免疫吸附测定，免疫荧光抗体测试确定，IFN-γ应答通过酶联免疫吸附点（ELIspot）确定。两种疫苗制剂在两个物种中都是高度免疫原性的。小鼠对C和R多肽的抗体反应更好，而N多肽在猴子中的免疫原性更高。抗肽抗体可检测数月之久，并能识别子孢子上的天然蛋白质。 Montanide ISA 720和Montanide ISA 51配方之间的差异不明显。

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