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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Genetic diversity of Plasmodium vivax Pvcsp and Pvmsp1 in Guyana, South America.
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Genetic diversity of Plasmodium vivax Pvcsp and Pvmsp1 in Guyana, South America.

机译：南美洲圭亚那间日疟原虫Pvcsp和Pvmsp1的遗传多样性。

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摘要

Approximately 55% of malaria infections in the Guyana Amazon region are attributed to Plasmodium falciparum while the other 45% are attributed to non-falciparum, mostly Plasmodium vivax. However, little is known about the P. vivax strain types circulating in the region. Using PCR for Plasmodium detection and two genetic markers specific to P. vivax to detect the polymorphic circumsporozoite protein (CSP) and the conserved 19-kDa region of the merozoite surface protein-1 (MSP-1), we investigated the overall Plasmodium strain distribution and population diversity within P. vivax in isolates collected from the blood of infected individuals in the interior Amazon region of Guyana, South America. Out of a total of 250 samples positive for Plasmodium, P. vivax was detected in 30% (76/250) and P. falciparum was detected in 76% (189/250). Mixed infections containing both P. falciparum and P. vivax constituted 6% (15/250) of the total positive samples. Further analysis of P. vivax strains showed that 92% (56/61) of the P. vivax samples hybridized with a probe specific to type VK210, 39% (24/61) hybridized with a probe specific for type VK247, and 25% (15/61) hybridized with a probe specific for the P. vivax-like CS genotype. DNA sequencing of the 19-kDa C-terminal domain in block 13 of MSP-1 amplified from 61 samples from patients infected with P. vivax demonstrated that this region is highly conserved, and all samples were identical at the nucleotide level to the Belem and Salvador-1 types. No synonymous or nonsynonymous mutations were observed in this region of the gene, indicating that current vaccine-development efforts based on the MSP-1(19) fragment would be applicable in Guyana.
机译：在圭亚那亚马逊地区，约55％的疟疾感染归因于恶性疟原虫，而其他45％归因于非恶性疟原虫，主要是间日疟原虫。但是，对该地区传播的间日疟原虫菌株类型了解甚少。使用PCR检测疟原虫和两个特定于间日疟原虫的遗传标记来检测多态环子孢子蛋白（CSP）和裂殖子表面蛋白1（MSP-1）的保守19-kDa区域，我们调查了整个疟原虫菌株的分布从南美圭亚那内陆亚马逊地区受感染者血液中分离得到的间日疟原虫的种群多样性。在总共250个疟原虫阳性样本中，间日疟原虫检出率为30％（76/250），恶性疟原虫检出率为76％（189/250）。包含恶性疟原虫和间日疟原虫的混合感染占阳性样本总数的6％（15/250）。对间日疟原虫菌株的进一步分析显示，间日疟原虫样品中有92％（56/61）与VK210型特异性探针杂交，39％（24/61）与VK247型特异性探针杂交，25％ （15/61）与间日疟原虫样CS基因型特异的探针杂交。从间日疟原虫感染患者的61份样本中扩增出的MSP-1区块13中19 kDa C末端结构域的DNA测序表明，该区域是高度保守的，并且所有样本在核苷酸水平上均与贝伦和Salvador-1类型。在该基因的该区域中未观察到同义或非同义突变，表明基于MSP-1（19）片段的当前疫苗开发工作将适用于圭亚那。

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