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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Evaluation of direct agglutination test, rk39 Test, and ELISA for the diagnosis of visceral leishmaniasis.
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Evaluation of direct agglutination test, rk39 Test, and ELISA for the diagnosis of visceral leishmaniasis.

机译：评估直接凝集试验，rk39试验和ELISA对内脏利什曼病的诊断。

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摘要

The study reports an evaluation of the direct agglutination test (DAT) with use of promastigote/amastigote antigen, rk39 strip test, and ELISA for diagnosis of visceral leishmaniasis (VL). Out of 94 clinically suspected VL patients, 16 (17%) were seropositive by all the techniques; in addition, 6 were positive in rk39 strip test and ELISA. On retrospective analysis, out of 16 positive by all the techniques, 11 (69%) had demonstrable Leishmania donovani (LD) bodies in their bone marrow samples, while in 5 bone marrow was not examined. Out of 6 that were positive by ELISA and rk39 strip test, 2 had myelofibrosis and 4 had chronic myeloid leukemia. On the basis of bone marrow aspirate positivity, the sensitivity and specificity of DAT were 100% while those of rk39 strip test and ELISA were 100% and 87%, respectively. The study suggests that DAT appears to be the best technique for the serodiagnosis of VL.
机译：这项研究报告了使用前鞭毛体/鞭毛体抗原，rk39剥离试验和ELISA诊断内脏利什曼病（VL）的直接凝集试验（DAT）的评估。在94例临床可疑的VL患者中，有16例（17％）通过所有方法血清阳性。另外，rk39试纸条和ELISA中有6例阳性。通过回顾性分析，在所有技术中阳性的16例中，有11例（69％）的骨髓样本中有可证实的利什曼原虫（LD）体，而没有检查5例骨髓。 ELISA和rk39试纸检测呈阳性的6例中，2例发生了骨髓纤维化，4例发生了慢性髓样白血病。根据骨髓抽吸物阳性，DAT的敏感性和特异性分别为100％，而rk39剥离试验和ELISA的敏感性和特异性分别为100％和87％。研究表明，DAT似乎是VL血清学诊断的最佳技术。

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