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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Molecular mass screening to incriminate sand fly vectors of Andean-type cutaneous leishmaniasis in Ecuador and Peru.
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Molecular mass screening to incriminate sand fly vectors of Andean-type cutaneous leishmaniasis in Ecuador and Peru.

机译：分子筛查确定厄瓜多尔和秘鲁的安第斯型皮肤利什曼病的沙蝇媒介。

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摘要

Sand flies from the Andean areas of Ecuador and Peru were examined for Leishmania infections by using our recently established molecular mass screening method. Leishmanial minicircle DNA-positive sand flies were detected in 3 of 192 and 1 of 462 samples from Ecuador and Peru, respectively. Sand fly species were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 18S ribosomal RNA (rRNA) gene, and the positive flies were Lutzomyia (Lu.) ayacuchensis and Lu. peruensis, respectively. Furthermore, cytochrome b and mannose-phosphate isomerase gene sequence analyses identified the parasites from Ecuador and Peru as Leishmania (Leishmania) mexicana and L. (Viannia) peruviana, respectively. Thus, the mass screening method was confirmed to be a powerful tool for sand fly research.
机译：厄瓜多尔和秘鲁安第斯地区的沙蝇使用我们最近建立的分子筛查方法检查了利什曼原虫感染。在分别来自厄瓜多尔和秘鲁的192个样本中有3个和462个样本中有1个检测到利什曼小圆环DNA阳性沙蝇。通过18S核糖体RNA（rRNA）基因的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）鉴定沙蝇种，阳性果蝇为琉球藻（Lu.omy ayacuchensis）和Lu。秘鲁。此外，细胞色素b和甘露糖磷酸异构酶基因序列分析确定了来自厄瓜多尔和秘鲁的寄生虫分别为利什曼原虫（Leishmania）mexicana和L.（Viannia）peruviana。因此，大量筛选方法被证实是沙蝇研究的有力工具。

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